Induced sputum methodology: Validity and reproducibility of total glutathione measurement in supernatant of healthy and asthmatic individuals

The Journal of Laboratory and Clinical Medicine
Jutta BeierRoland Buhl

Abstract

Glutathione (GSH), a major antioxidant, has repeatedly been linked to the pathogenesis of pulmonary disease. The measurement of GSH in induced sputum (IS) offers a noninvasive tool for the study and monitoring of oxidative stress in airway diseases. In this study we assessed the validity and reproducibility of GSH quantification in IS from healthy subjects and individuals with mild asthma. We spectrophotometrically quantified total GSH in the IS of 31 healthy nonsmoking volunteers and 12 individuals with mild asthma. IS was processed with varying concentrations of dithiothreitol (DTT) in an effort to evaluate the effect of DTT on GSH measurements. We performed spiking experiments with defined concentrations of GSH and quantified the percentage of recovery and also analyzed the effect of induction time on GSH levels through sequential sampling of sputum portions (15, 30, and 45 minutes' induction). Finally we tested the reproducibility of GSH measurements at 2 separate time points (0 and 72 hours) and expressed it as an intraclass correlation coefficient (R(i)) with a coefficient of reliability (CR). Processing with DTT increased GSH values in IS (P <.05 for each DTT concentration > 0.001%). Recovery of GSH after spiking was com...Continue Reading

References

Feb 1, 1989·The American Review of Respiratory Disease·A M CantinR G Crystal
Apr 1, 1995·The Journal of Allergy and Clinical Immunology·J V FahyH A Boushey
Jun 1, 1993·The American Review of Respiratory Disease·L J SmithJ Anderson
Aug 1, 1996·American Journal of Respiratory and Critical Care Medicine·E PizzichiniF E Hargreave
Feb 1, 1997·American Journal of Respiratory and Critical Care Medicine·V M KeatingsP J Barnes
May 30, 1998·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·O HolzH Magnussen
May 8, 1999·The European Respiratory Journal·R LouisR Djukanovic
Nov 11, 1999·American Journal of Respiratory and Critical Care Medicine·S V CulpittP J Barnes
Jul 6, 2000·The Journal of Laboratory and Clinical Medicine·R E NockerJ S van der Zee
Sep 1, 2000·The European Respiratory Journal·D LoppowR A Jörres
Sep 1, 2000·The European Respiratory Journal·O HolzH Magnussen
Sep 19, 2000·Thorax·R Dworski
Nov 22, 2001·The European Respiratory Journal·M M KellyG Cox
Dec 6, 2001·American Journal of Respiratory and Critical Care Medicine·N E AlexisW D Bennett
Apr 1, 1964·Biochemistry·W W CLELAND

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Citations

Feb 7, 2012·Antioxidants & Redox Signaling·Anne M FitzpatrickLou Ann S Brown
Oct 11, 2007·Chest·Claudia CostaLouise E Donnelly
Feb 18, 2016·Oxidative Medicine and Cellular Longevity·Balazs Antus
Feb 2, 2011·Biochimica Et Biophysica Acta·Niki L Reynaert
Mar 18, 2008·Free Radical Biology & Medicine·Marina E SolovievaVladimir S Akatov
Feb 1, 2008·Clinical Nutrition : Official Journal of the European Society of Parenteral and Enteral Nutrition·Lisa G WoodPeter G Gibson
Aug 30, 2014·Respirology : Official Journal of the Asian Pacific Society of Respirology·Jing ZhangWanzhen Yao
Mar 13, 2014·Proteomics. Clinical Applications·Elizabeth V NguyenDavid R Goodlett
Nov 3, 2016·Biological Trace Element Research·Hacer DoganFikret Karatas
May 18, 2016·Analytical Chemistry·Sixing LiTony Jun Huang
Jun 18, 2015·Lab on a Chip·Po-Hsun HuangTony Jun Huang
May 23, 2007·Journal of Cellular Physiology·Prisco MirandolaMarco Vitale
Jul 2, 2018·Journal of Cystic Fibrosis : Official Journal of the European Cystic Fibrosis Society·O J McElvaneyN G McElvaney

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