Inducement of cytopathic changes and plaque formation by porcine haemagglutinating encephalomyelitis virus

Veterinary Microbiology
K SatoM Matumoto

Abstract

ESK cells were shown to be a good medium for propagating the 67N strain of porcine haemagglutinating encephalomyelitis virus, although no cytopathic effect was observed. The virus induced a readily recognizable cytopathic effect in ESK cells, when a noncytotoxic amount of diethylaminoethyl-dextran (DEAE-dextran) was incorporated in the culture medium. Based on this finding, a sensitive, practical assay method for the virus was developed. When DEAE-dextran was incorporated in the agar overlay medium, 67N virus formed plaques in ESK cell monolayers. The cytopathic effect as well as the plaque formation were specifically inhibited by antisera against the virus. Neutralization tests were developed on the basis of these findings. Neutralization and haemagglutination-inhibition tests on swine serum samples indicated a wide dissemination of haemagglutinating encephalomyelitis virus or antigenically-related viruses in Japanese pigs.

References

Aug 1, 1967·Proceedings of the Society for Experimental Biology and Medicine·A VaheriS A Plotkin
Jan 1, 1965·Archiv für die gesamte Virusforschung·J S Pagano, A Vaheri
Jan 1, 1969·Archiv für die gesamte Virusforschung·A F Bradburne, D A Tyrrell
Feb 1, 1981·Proceedings of the Society for Experimental Biology and Medicine·K SasakiS A Plotkin

Citations

Aug 1, 1996·Zentralblatt für Veterinärmedizin. Reihe B. Journal of veterinary medicine. Series B·N HiranoR Yamaguchi

Related Concepts

Antibodies, Viral
Coronaviridae
Cytopathogenic Effect, Viral
Pulsar
Embryonic Structures, Mammalian
Hemagglutination Inhibition Tests
Kidney
Neutralization Tests
Bacteriophage Plaque Assay
Virus Cultivation

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