PMID: 7029544Sep 1, 1981Paper

Inducibility of a gene product required for UV and chemical mutagenesis in Escherichia coli

Proceedings of the National Academy of Sciences of the United States of America
A BaggG C Walker

Abstract

The product of the umuC gene is required for UV and chemical mutagenesis in Escherichia coli. By the use of the Mud(Ap, lac) bacteriophage, we have obtained an operon fusion of the lac structural genes to the promoter/regulatory region of the umuC gene. The strain containing the umuC::Mud(Ap, lac) fusion was identified on the basis of its UV nonmutability. Strains containing this putative null allele of umuC were (i) nonmutable by UV and other agents, (ii) slightly UV sensitive, and (iii) deficient in their ability to carry out Weigle reactivation of UV-irradiation bacteriophage lambda. The UV nonmutability of the strain could be suppressed by a derivative of the mutagenesis-enhancing plasmid pKM101. beta-Galactosidase synthesis in umuC::Mud(Ap, lac) fusion strains was inducible by UV and other DNA-damaging agents. Genetic analysis of the regulation of beta-galactosidase in umuC::Mud(Ap, lac) strains suggests that the lexA protein is the direct repressor of the umuC gene and that a function of the recA protein, probably its protease activity, is required for the removal of the lexA repressor at the time of umuC induction.

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Citations

Feb 9, 2002·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Martín Gonzalez, Roger Woodgate
May 1, 1991·Molecular & General Genetics : MGG·A QuiñonesW Messer
Oct 1, 1989·Molecular & General Genetics : MGG·M KaaschA Quiñones
Oct 1, 1991·Molecular & General Genetics : MGG·S G SedgwickP Strike
Jan 1, 1983·Molecular & General Genetics : MGG·R G Lloyd
Jan 1, 1984·Molecular & General Genetics : MGG·B J Kilbey
Jan 1, 1983·Molecular & General Genetics : MGG·R G LloydC Prescott
Jan 1, 1986·Molecular & General Genetics : MGG·R PiechockiR Langhammer
Jan 1, 1984·Molecular & General Genetics : MGG·S B DowdenP Strike
Jan 1, 1982·Molecular & General Genetics : MGG·C E Shurvinton, R G Lloyd
Aug 1, 1989·Molecular & General Genetics : MGG·S G SedgwickP Strike
Jan 1, 1982·Molecular & General Genetics : MGG·Z Cieśla
Feb 1, 1987·Molecular & General Genetics : MGG·M L Morse, D S Smith
Jan 1, 1982·Molecular & General Genetics : MGG·S CasaregolaO Huisman
Jan 1, 1982·Molecular & General Genetics : MGG·G EitnerJ Hofemeister
Jan 1, 1983·Molecular & General Genetics : MGG·R DevoretP L Moreau
Aug 1, 1988·Molecular & General Genetics : MGG·D G EnnisD W Mount
Jan 1, 1983·Molecular & General Genetics : MGG·M Defais
Jan 1, 1982·Molecular & General Genetics : MGG·S B Dowden, P Strike
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Nov 1, 1986·Molecular & General Genetics : MGG·M BlancoV Aleixandre
Jan 25, 2012·Biochemical Genetics·Swati Saha, Tarakdas Basu
Dec 1, 2010·Environmental Monitoring and Assessment·Athar Habib SiddiquiMasood Ahmad
Nov 1, 1982·Mutation Research·R M SchaaperL A Loeb
Jan 1, 1984·Mutation Research·P F Schendel, I Michaeli
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May 1, 1985·Mutation Research·I PietrzykowskaD Shugar
Jun 1, 1987·Mutation Research·L Shirnamé-MoréK Frenkel
Sep 1, 1990·Mutation Research·G von PoserJ A Henriques

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