Inducible cell-to-cell signaling for tunable dynamics in microbial communities.

Nature Communications
Arianna MianoJeff Hasty

Abstract

The last decade has seen bacteria at the forefront of biotechnological innovation, with applications including biomolecular computing, living therapeutics, microbiome engineering and microbial factories. These emerging applications are all united by the need to precisely control complex microbial dynamics in spatially extended environments, requiring tools that can bridge the gap between intracellular and population-level coordination. To address this need, we engineer an inducible quorum sensing system which enables precise tunability of bacterial dynamics both at the population and community level. As a proof-of-principle, we demonstrate the advantages of this system when genetically equipped for cargo delivery. In addition, we exploit the absence of cross-talk with respect to the majority of well-characterized quorum sensing systems to demonstrate inducibility of multi-strain communities. More broadly, this work highlights the unexplored potential of remotely inducible quorum sensing systems which, coupled to any gene of interest, may facilitate the translation of circuit designs into applications.

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Citations

Feb 13, 2021·Trends in Biotechnology·Ivan SchlembachLars Regestein
Dec 9, 2020·International Journal of Molecular Sciences·Alvaro BanderasPascal Hersen
Nov 18, 2020·Current Opinion in Biotechnology·Kent M RappMichael J Betenbaugh
May 27, 2021·Nature Communications·Ximing LiRamez Daniel
May 23, 2021·Trends in Microbiology·Shengbo WuJianjun Qiao
Jul 9, 2021·Nature Reviews. Genetics·Monica P McNerneyPamela A Silver
Oct 7, 2021·Nature Communications·Chetan AdityaJakob Ruess

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Methods Mentioned

BETA
fluorescence microscopy
chip
PCR

Software Mentioned

iQS
Nikon Elements
ImageJ
Matlab

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