Inducible Cre transgenic mouse strain for skeletal muscle-specific gene targeting.

Skeletal Muscle
John J McCarthyKaryn A Esser

Abstract

The use of the Cre/loxP system for gene targeting has been proven to be a powerful tool for understanding gene function. The purpose of this study was to create and characterize an inducible, skeletal muscle-specific Cre transgenic mouse strain. To achieve skeletal muscle-specific expression, the human α-skeletal actin promoter was used to drive expression of a chimeric Cre recombinase containing two mutated estrogen receptor ligand-binding domains. Western blot analysis, PCR and β-galactosidase staining confirmed that Cre-mediated recombination was restricted to limb and craniofacial skeletal muscles only after tamoxifen administration. A transgenic mouse was created that allows inducible, gene targeting of floxed genes in adult skeletal muscle of different developmental origins. This new mouse will be of great utility to the skeletal muscle community.

References

Jan 22, 1998·The American Journal of Physiology·T CollinsE C Hardeman
Sep 11, 1999·Nucleic Acids Research·P MiniouJ Melki
Oct 2, 2003·Biology of the Cell·Onnik AgbulutGillian Butler-Browne
Jan 14, 2005·Proceedings of the National Academy of Sciences of the United States of America·Shijie LiEric N Olson
Feb 25, 2005·Genesis : the Journal of Genetics and Development·Jennifer C J ChenDavid J Goldhamer
Mar 25, 2005·Genesis : the Journal of Genetics and Development·Michael SchulerDaniel Metzger
Feb 15, 2007·Proceedings of the National Academy of Sciences of the United States of America·Masanori TakehashiTakashi Shinohara
Apr 10, 2007·Cancer Cell·Malay HaldarMario R Capecchi
Jan 24, 2009·Genesis : the Journal of Genetics and Development·Masakazu YamamotoDavid J Goldhamer
Mar 6, 2009·Developmental Neurobiology·Pengcheng Rao, D Ashley Monks
Apr 1, 2009·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Koichi NishijoCharles Keller
May 26, 2009·Developmental Biology·Onur KanisicakDavid J Goldhamer
Dec 24, 2010·Development·Sam J MathewGabrielle Kardon
Aug 11, 2011·Development·John J McCarthyCharlotte A Peterson

❮ Previous
Next ❯

Citations

Oct 22, 2015·The Journal of Physiology·Elizabeth A SchroderKaryn A Esser
Mar 26, 2013·Biochimica Et Biophysica Acta·Mark RomanickHolly M Brown-Borg
Jun 2, 2016·The Journal of Physiology·Elizabeth SchroderKaryn Esser
Oct 27, 2016·American Journal of Physiology. Endocrinology and Metabolism·Christopher BallmannGlenn C Rowe
Feb 9, 2018·JCI Insight·Sajedah M HindiAshok Kumar
Feb 7, 2018·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Kathryn C JacksonEspen E Spangenburg
Mar 8, 2018·Journal of Cachexia, Sarcopenia and Muscle·Zong-Kang ZhangBao-Ting Zhang
Sep 29, 2017·Shock·Orlando LaitanoElizabeth A Nunamaker
Jul 21, 2017·ELife·J Christopher EhlenKetema N Paul
Aug 29, 2018·American Journal of Physiology. Endocrinology and Metabolism·Kristoffer SvenssonSimon Schenk
Sep 12, 2018·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Yann S GallotAshok Kumar
Sep 28, 2018·Journal of Applied Physiology·Margaret B BellGlenn C Rowe
Dec 5, 2018·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Jae-Sung YouTroy A Hornberger
Jun 22, 2019·Journal of Cachexia, Sarcopenia and Muscle·Christian M GirgisJenny E Gunton
Jul 3, 2019·American Journal of Physiology. Endocrinology and Metabolism·Jens Frey HallingHenriette Pilegaard
Nov 8, 2019·Journal of Cachexia, Sarcopenia and Muscle·Alexander S HamMarkus A Rüegg
Jan 4, 2020·Journal of Cachexia, Sarcopenia and Muscle·Kristoffer SvenssonSimon Schenk
Dec 4, 2019·American Journal of Physiology. Endocrinology and Metabolism·Kristoffer SvenssonSimon Schenk
Nov 13, 2015·BMC Developmental Biology·Mingsen LiDelin Mo
Sep 3, 2016·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Ellie M CarrellRobert T Dirksen
Feb 28, 2020·Journal of Applied Physiology·C Brooks MobleyJohn J McCarthy
Mar 12, 2020·Muscle & Nerve·Andrea L Reid, Matthew S Alexander
May 15, 2020·Nature Metabolism·Anthony R P VerkerkeKatsuhiko Funai
May 23, 2020·The Journal of Experimental Medicine·Aneesha DasguptaPankaj K Singh
Jul 17, 2020·Journal of Applied Physiology·Kevin A MurachCory M Dungan
Sep 8, 2020·The Journal of Physiology·Lie GaoIrving H Zucker
May 12, 2018·Diabetes·Dmitri SamovskiNada A Abumrad
Mar 20, 2019·American Journal of Physiology. Endocrinology and Metabolism·Vitor F MartinsSimon Schenk
May 7, 2020·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Bjørg Thiellesen BuchHenriette Pilegaard
Jul 20, 2019·Proceedings of the National Academy of Sciences of the United States of America·Julien DelezieChristoph Handschin
Aug 2, 2017·Annals of the New York Academy of Sciences·Christopher P Cardozo, Zachary A Graham
Jun 1, 2014·Clinical Reviews in Bone and Mineral Metabolism·Robert D Blank

❮ Previous
Next ❯

Methods Mentioned

BETA
transgenic
biopsies
PCR
genotyping
protein assay
X-ray

Software Mentioned

SPOT RT

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.