Inducible expression of chimeric EWS/ETS proteins confers Ewing's family tumor-like phenotypes to human mesenchymal progenitor cells.

Molecular and Cellular Biology
Yoshitaka MiyagawaNobutaka Kiyokawa

Abstract

Ewing's family tumor (EFT) is a rare pediatric tumor of unclear origin that occurs in bone and soft tissue. Specific chromosomal translocations found in EFT cause EWS to fuse to a subset of ets transcription factor genes (ETS), generating chimeric EWS/ETS proteins. These proteins are believed to play a crucial role in the onset and progression of EFT. However, the mechanisms responsible for the EWS/ETS-mediated onset remain unclear. Here we report the establishment of a tetracycline-controlled EWS/ETS-inducible system in human bone marrow-derived mesenchymal progenitor cells (MPCs). Ectopic expression of both EWS/FLI1 and EWS/ERG proteins resulted in a dramatic change of morphology, i.e., from a mesenchymal spindle shape to a small round-to-polygonal cell, one of the characteristics of EFT. EWS/ETS also induced immunophenotypic changes in MPCs, including the disappearance of the mesenchyme-positive markers CD10 and CD13 and the up-regulation of the EFT-positive markers CD54, CD99, CD117, and CD271. Furthermore, a prominent shift from the gene expression profile of MPCs to that of EFT was observed in the presence of EWS/ETS. Together with the observation that EWS/ETS enhances the ability of cells to invade Matrigel, these result...Continue Reading

References

Sep 1, 1989·Japanese Journal of Cancer Research : Gann·C HommaM Sakurai
Dec 1, 1989·British Journal of Cancer·S HaraJ Hata
Jun 15, 1982·International Journal of Cancer. Journal International Du Cancer·J WielsT Tursz
Sep 1, 1982·Cancer Genetics and Cytogenetics·F GilbertH Schlesinger
Jan 1, 1995·Virchows Archiv : an International Journal of Pathology·A OgoseH Watanabe
Dec 17, 1997·Nature Genetics·W A MayC T Denny
Aug 24, 1999·Journal of Cellular Physiology·P A Conget, J J Minguell
Dec 10, 1999·Proceedings of the National Academy of Sciences of the United States of America·R S HubertD E Afar
Aug 10, 2000·Journal of Neuroscience Research·D WoodburyI B Black
Jun 28, 2001·Proceedings of the National Academy of Sciences of the United States of America·D C ColterD J Prockop
Jul 19, 2001·Annals of the New York Academy of Sciences·M Reyes, C M Verfaillie
Oct 19, 2001·Oncogene·A Arvand, C T Denny
Jul 24, 2002·Experimental Hematology·Nadia QuiriciGiorgio Lambertenghi Deliliers
Dec 17, 2002·Arthritis and Rheumatism·Elena A JonesDennis McGonagle
Jan 2, 2003·Molecular and Cellular Biology·Susan EliazerRobert L Ilaria
May 16, 2003·Molecular and Cellular Biology·Benjamin DeneenChristopher T Denny
Feb 5, 2004·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Iranzu GonzálezEnrique de Alava
Nov 3, 2004·Trends in Molecular Medicine·Tsuyoshi Akagi
Jun 2, 2005·Insect Molecular Biology·Y MiyagawaT Kusakabe
Aug 13, 2005·Advances in Anatomic Pathology·Joseph D Khoury
May 16, 2006·Cancer Cell·Sean Davis, Paul S Meltzer
Nov 2, 2006·The Tohoku Journal of Experimental Medicine·Masahito HatoriShoichi Kokubun

❮ Previous
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Citations

Mar 1, 2012·Archives of Pharmacal Research·Ji-Seon LeeHyuk-Jin Cha
Jun 15, 2010·Oncogene·E C ToomeyS L Lessnick
Mar 18, 2009·Proceedings of the National Academy of Sciences of the United States of America·Günther H S RichterStefan Burdach
Oct 19, 2010·Sarcoma·Patrick P LinGuillermina Lozano
Jan 28, 2010·Cancer Research·Eun Jung SohnSean Bong Lee
Mar 4, 2010·The Journal of Clinical Investigation·Anna RocchiKatia Scotlandi
Jul 25, 2013·Clinical Sarcoma Research·Wei XiaoAnne-Marie Cleton-Jansen
Oct 8, 2011·Disease Models & Mechanisms·Stefanie W LeacockJames F Amatruda
Feb 28, 2009·PloS One·Yoshitaka MiyagawaNobutaka Kiyokawa
May 12, 2011·PloS One·Cornelia von LevetzowElizabeth R Lawlor
Dec 18, 2010·Pediatric and Developmental Pathology : the Official Journal of the Society for Pediatric Pathology and the Paediatric Pathology Society·Bekim SadikovicGino R Somers
May 11, 2012·Pediatric and Developmental Pathology : the Official Journal of the Society for Pediatric Pathology and the Paediatric Pathology Society·Maria TsokosPaul S Dickman
Jul 18, 2014·Virchows Archiv : an International Journal of Pathology·Ryo ShibuyaMasanori Hisaoka
Oct 18, 2008·Expert Review of Anticancer Therapy·Susan Ann Burchill
Jul 21, 2015·International Journal of Molecular Sciences·Laurens G L SandPancras C W Hogendoorn
May 6, 2015·Applied Microbiology and Biotechnology·Takao KitagawaKazuyuki Nakamura
Jan 8, 2013·Cancer Biology & Therapy·Dorothea E Mahlendorf, Martin Sebastian Staege
Aug 12, 2014·Advanced Drug Delivery Reviews·Salah-Eddine Lamhamedi-CherradiJoseph A Ludwig
Jan 19, 2013·Seminars in Diagnostic Pathology·Alison L CheahSteven D Billings
Mar 27, 2012·The American Journal of Surgical Pathology·Akihiko YoshidaHitoshi Tsuda
Jan 7, 2018·Journal of Cell Communication and Signaling·Michela PaselloKatia Scotlandi
Apr 26, 2008·Pediatric Blood & Cancer·Juergen L FoellMartin S Staege
Nov 1, 2008·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Yuko SegawaIchiro Sekiya
Aug 25, 2009·Advances in Anatomic Pathology·John A Ozolek
Oct 11, 2011·The Journal of Pathology·Idriss M Bennani-BaitiHeinrich Kovar
Nov 10, 2009·The Oncologist·Melinda S Merchant, Crystal L Mackall
Nov 24, 2012·Seminars in Cutaneous Medicine and Surgery·Alison L Cheah, Steven D Billings
Apr 20, 2019·Frontiers in Immunology·Elisabeth J M HuijbersArjan W Griffioen

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