Inducible nitric oxide synthase and cyclooxgenase-2 mediate protection of hydrogen peroxide preconditioning against apoptosis induced by oxidative stress in PC12 cells

Life Sciences
Xiao-qing TangPei-xi Chen

Abstract

The induction of inducible nitric oxide synthase (iNOS) in response to different stress is associated with simultaneous induction of cyclooxygenase-2 (COX-2) in various cell types. Both iNOS and COX-2 have been reported to mediate the late phase of cardioprotection induced by different preconditioning. However, whether both iNOS and COX-2 are mediators in the neuroprotection induced by preconditioning with hydrogen peroxide (H(2)O(2)) at low concentration is unknown. In this study, using the neurosecretory cell line-PC12 cells to set up the model of neuroprotection of preconditioning with H(2)O(2) against apoptosis, we first investigate what changes in expression of iNOS and COX-2 appear during H(2)O(2) preconditioning, then determine if both iNOS inhibitor and COX-2 inhibitor interfere with the neuroprotection elicited by preconditioning with H(2)O(2). We found that preconditioning with H(2)O(2) at 10 microM significantly protected PC12 cells against apoptosis induced by lethal H(2)O(2) (50 microM) and increased the expression of iNOS and COX-2 and that selective iNOS inhibitor, aminoguanidine (AG) and COX-2 inhibitor, NS-398 obviously blocked the protective effects induced by preconditioning with 10 microM H(2)O(2). The resul...Continue Reading

References

Dec 27, 1996·The Journal of Biological Chemistry·W L SmithD L DeWitt
May 23, 1998·Annual Review of Pharmacology and Toxicology·J R VaneR M Botting
Sep 29, 1999·Proceedings of the National Academy of Sciences of the United States of America·Y GuoR Bolli
Aug 30, 2000·Proceedings of the National Academy of Sciences of the United States of America·K ShinmuraR Bolli
Nov 25, 2000·Circulation Research·R Bolli
Oct 18, 2002·American Journal of Physiology. Heart and Circulatory Physiology·Eitaro KodaniRoberto Bolli
Oct 22, 2002·American Journal of Physiology. Heart and Circulatory Physiology·Ken ShinmuraRoberto Bolli
Jun 12, 2003·Cardiovascular Research·Claire ArnaudChristophe Ribuot
May 4, 2004·Life Sciences·Hemal H PatelGarrett J Gross

❮ Previous
Next ❯

Citations

Nov 15, 2011·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Yaghub PazhangMahshid Shafiezadeh
Nov 30, 2011·Antioxidants & Redox Signaling·Rommy von Bernhardi, Jaime Eugenín
Feb 15, 2008·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Donna M WilcockCarol A Colton
Sep 2, 2008·Brain Research·Rajesh K SharmaPeter A Netland
May 15, 2009·Free Radical Biology & Medicine·Richard D BrownGuy C Brown
Feb 9, 2008·Cell Death and Differentiation·G Loor, P T Schumacker
Apr 12, 2011·American Journal of Physiology. Heart and Circulatory Physiology·Cristina AngeloniSilvana Hrelia
Dec 19, 2016·Pflügers Archiv : European journal of physiology·Rossana MorabitoAngela Marino
Nov 6, 2020·ACS Chemical Neuroscience·Rachid AkkiHicham Mohti

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis