PMID: 9169863May 15, 1997Paper

Inducible nitric oxide synthase and nitric oxide production by oligodendrocytes

Journal of Neuroscience Research
J E MerrillC J Lowenstein

Abstract

It has been previously demonstrated that microglia and astrocytes produce micromolar amounts of nitric oxide in vitro. In this study, we demonstrate that primary rat oligodendrocytes can be stimulated to produce iNOS mRNA as detected by Northern blot and in situ hybridization analysis and a 131-kDa iNOS protein by Western blot analysis; protein was also detected in cells by single- and double-label immunohistochemistry for iNOS and the oligodendrocyte-specific marker CNPase. NO/NOS are produced as a consequence of activation of the gene encoding the inducible nitric oxide synthase as determined by inhibition with actinomycin D and cyclohexamide. The iNOS is functional, leading to calcium/calmodulin-independent NO production in these in vitro cultures.

Citations

Jul 7, 2005·Proceedings of the National Academy of Sciences of the United States of America·Jianrong LiPaul A Rosenberg
Aug 22, 2008·Antioxidants & Redox Signaling·Andrew B Knott, Ella Bossy-Wetzel
Jan 13, 2000·Neuropathology and Applied Neurobiology·J E Merrill, N J Scolding
Oct 31, 2002·The European Journal of Neuroscience·Carme SolàJoan Serratosa
Sep 19, 2012·Cytokine & Growth Factor Reviews·Pía M VidalSven Hendrix
Jun 30, 2006·Journal of Neurochemistry·Salvador Moncada, Juan P Bolaños
Mar 6, 2002·British Journal of Pharmacology·Juan V Esplugues

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