Inducible nitric oxide synthase (iNOS) in pancreatic islets of nonobese diabetic mice: identification of iNOS- expressing cells and relationships to cytokines expressed in the islets

Endocrinology
A RabinovitchR C Bleackley

Abstract

Inflammatory cytokines and nitric oxide (NO) are candidate mediators of pancreatic islet beta-cell destruction in insulin-dependent diabetes mellitus. In this study, we used a semiquantitative PCR assay to measure levels of messenger RNA (mRNA) expression of the inflammatory cytokines, interleukin-1 alpha (IL-1 alpha), tumor necrosis factor-alpha, and interferon-gamma (IFN gamma), and of the inducible form of NO synthase (iNOS) in mononuclear leukocytes isolated from pancreatic islets of autoimmune diabetes-prone nonobese diabetic (NOD) female mice. We found that mRNA levels of iNOS, IL-1 alpha, and IFN gamma in islet mononuclear leukocytes increased from 5 weeks of age to onset of diabetes ( > 13 weeks of age). To determine whether increased iNOS, IL-1 alpha, and IFN gamma mRNA expressions were related to diabetes development, we compared mRNA levels of these molecules in mononuclear leukocytes from islets of 12 week-old diabetes-prone NOD female mice and three groups of 12-week-old mice with low diabetes risk: NOD female mice injected with complete Freund's adjuvant at 4 weeks of age, NOD male mice, and BALB/c female mice that do not develop diabetes. We found that iNOS, IL-1 alpha, and IFN gamma mRNA levels were higher in mo...Continue Reading

Citations

May 27, 1997·Proceedings of the National Academy of Sciences of the United States of America·C P RobinsonM G Humphreys-Beher
Oct 19, 1999·The Journal of Experimental Medicine·S T GreyC Ferran
Jun 17, 2004·Molecular and Cellular Biology·Matthew G HartmanTsonwin Hai
Jan 24, 2007·Physiological Reviews·Pál PacherLucas Liaudet
Aug 6, 1998·The Journal of Clinical Investigation·M ArnushJ A Corbett
Feb 22, 2001·Biochemical and Biophysical Research Communications·A K AnderssonS Sandler
Aug 23, 2001·International Immunopharmacology·K D KrönckeV Kolb-Bachofen
Sep 15, 2001·Mechanisms of Ageing and Development·K WatanabeY Iwatani
Jan 29, 2011·Apoptosis : an International Journal on Programmed Cell Death·T SatohK Eguchi
Aug 12, 1998·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·T M Fonovich de SchroederO L Catanzaro
Nov 12, 2002·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·D Ventura-OliveiraR L Zollner
Jul 18, 2000·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·M YamakawaG R Login
Dec 18, 2003·Annals of the New York Academy of Sciences·Kathryn HaskinsJennifer Kench
Feb 3, 2007·Cell Transplantation·Andreas BörjessonStellan Sandler
Feb 9, 2005·Annals of the New York Academy of Sciences·Shiva Reddy, Joshua Bradley
Apr 3, 2008·Cell Biochemistry and Function·Maurício da Silva Krause, Paulo Ivo Homem de Bittencourt
Aug 10, 2000·Diabetes/metabolism Research and Reviews·H E Thomas, T W Kay
Sep 10, 2013·Molecular Endocrinology·Susan J BurkeJ Jason Collier
Dec 4, 2001·Journal of Investigative Medicine : the Official Publication of the American Federation for Clinical Research·E TobiaschF H Bach
Nov 30, 2000·Upsala Journal of Medical Sciences·S SandlerN Welsh
Feb 15, 2001·The International Journal of Angiology : Official Publication of the International College of Angiology, Inc·Jörg BojungaKlaus Kusterer
Jul 9, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Israel I MendezJi-Won Yoon
May 17, 2008·Biochemical Society Transactions·Danielle Melloul
Jun 30, 2000·Biochemical and Biophysical Research Communications·T TabatabaieY Kotake
May 4, 2021·Critical Reviews in Food Science and Nutrition·Susmita Barman, Krishnapura Srinivasan
Aug 27, 1998·Biochemical Pharmacology·A Rabinovitch, W L Suarez-Pinzon

❮ Previous
Next ❯

Related Concepts

Related Feeds

Autoimmune Diabetes & Tolerance

Patients with type I diabetes lack insulin-producing beta cells due to the loss of immunological tolerance and autoimmune disease. Discover the latest research on targeting tolerance to prevent diabetes.