PMID: 15220775Jun 29, 2004Paper

Inducible nitric oxide synthase mediates delayed cardioprotection induced by morphine in vivo: evidence from pharmacologic inhibition and gene-knockout mice

Anesthesiology
Xiaojing JiangShigehito Sato

Abstract

It is not known whether morphine induces delayed cardioprotection against ischemia and reperfusion. The authors measured the delayed preconditioning induced by morphine and determined the role of inducible nitric oxide synthase (iNOS) in mediating this effect using a pharmacological inhibitor and iNOS gene-knockout mice. Adult male wild-type and iNOS gene-knockout (B6, 129) mice were treated with morphine (0.3 or 0.1 mg/kg intraperitoneal) or saline. Twenty-four hours later, mice were subjected to 45 min of coronary artery occlusion followed by 120 min of reperfusion. S-methylthiourea sulfate (3 mg/kg, intraperitoneal) was given 30 min before the occlusion to block iNOS. Infarct size as a percentage of the area at risk was determined by triphenyltetrazolium chloride staining. iNOS and endothelial nitric oxide synthase expression were measured by Western blot. Infarct size was significantly reduced in wild-type mice from 43.1 +/- 5.3% in the saline group to 22.4 +/- 4.4% in the higher-dose morphine group (0.3 mg/kg) (P < 0.05). This cardioprotective effect was abolished by S-methylthiourea sulfate (43.3 +/- 3.9%) and was absent in iNOS gene-knockout mice (42.3 +/- 4.7%). Pretreatment with the lower dose of morphine (0.1 mg/kg) d...Continue Reading

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Citations

Nov 19, 2009·Journal of Anesthesia·Noboru TodaHiroshi Toda
May 25, 2010·Cardiovascular Drugs and Therapy·Derek J Hausenloy, Derek M Yellon
Jul 12, 2005·American Journal of Physiology. Heart and Circulatory Physiology·Yiru GuoRoberto Bolli
Dec 19, 2014·British Journal of Pharmacology·John P HeadrickJason N Peart
Jun 6, 2006·Life Sciences·Seyedmehdi PayabvashAhmad R Dehpour
Jul 26, 2005·Peptides·Richard J Bodnar, Gad E Klein
Jun 1, 2005·Vascular Pharmacology·Jason N PeartGarrett J Gross
Mar 25, 2015·Journal of Cardiothoracic and Vascular Anesthesia·Christopher Lotz, Franz Kehl
May 17, 2005·Journal of the American College of Cardiology·Xiaojing JiangHui Yue
Jul 6, 2010·Free Radical Biology & Medicine·Rouhollah HabibeyHamidreza Pazoki-Toroudi
Dec 13, 2005·American Journal of Physiology. Heart and Circulatory Physiology·Shaul AtarYochai Birnbaum
Oct 31, 2020·European Journal of Pharmacology·Li-Ning WuJun-Ma Yu

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