Inducible nitric oxide synthase mediates gut ischemia/reperfusion-induced ileus only after severe insults

The Journal of Surgical Research
Heitham T HassounFrederick A Moore

Abstract

Inducible nitric oxide synthase (NOS 2) is thought to play a role in gut motility disorders that occur under proinflammatory conditions. Clinically, ileus occurs after sepsis and shock-induced gut ischemia/reperfusion (I/R). The purpose of this study was to determine if NOS 2 mediates impaired intestinal transit in well-established models of both moderate and severe gut ischemia/reperfusion. At laparotomy, Sprague-Dawley rats had duodenal catheters placed. Small intestinal transit was determined by quantitating the percentage tracer (FITC-dextran) in 10 equal segments of intestine 30 min after catheter injection [expressed as the mean geometric center (MGC) of distribution]. Transit was assessed at 6 and 24 h after gut ischemia [45 or 75 min of superior mesenteric artery occlusion (SMAO) with sham laparotomy as control]. In a separate set of experiments, N(6)-(iminoethyl)-L-lysine (L-NIL), a selective NOS 2 antagonist, was administered 1 h prior to laparotomy and transit was determined after 6 h as described above. Ileal NOS 2 expression was assessed by Western immunoblot and quantitative "real-time" RT-PCR. We observed that both 45 and 75 min of SMAO decreased intestinal transit at 6 h of reperfusion compared to sham. Ileal NO...Continue Reading

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Citations

Apr 5, 2012·Scandinavian Journal of Trauma, Resuscitation and Emergency Medicine·Shinil K ShahCharles S Cox
Apr 4, 2008·Journal of Clinical Biochemistry and Nutrition·Atsunori NakaoKenneth R McCurry
Dec 2, 2015·Experimental and Therapeutic Medicine·Yong SunWen-Jie Fan
Dec 5, 2009·The Journal of Surgical Research·Shinil K ShahCharles S Cox
Nov 20, 2009·The Journal of Surgical Research·Güray SoydanMeral Tuncer
Jun 2, 2009·The Journal of Surgical Research·Stacey D Moore-OlufemiCharles S Cox
Jul 2, 2010·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·S K ShahC S Cox
Jan 3, 2013·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·C GiaroniG Frigo
Apr 20, 2004·The Journal of Surgical Research·B O AttuwaybiF A Moore
Jul 29, 2006·The Journal of Surgical Research·Naoki ShimojimaMasaki Kitajima
Jul 30, 2005·The Veterinary Clinics of North America. Equine Practice·Clare A Ryan, L Chris Sanchez
Jun 28, 2005·The Journal of Surgical Research·S D Moore-OlufemiC S Cox
Jan 8, 2004·Acta Physiologica Hungarica·M Boros
Aug 16, 2016·Journal of Cardiothoracic and Vascular Anesthesia·Tülün ÖztürkIşıl Aydemir
May 31, 2008·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·H Ługowska-UmerR P Korolkiewicz
Jan 1, 2008·JPEN. Journal of Parenteral and Enteral Nutrition·Margaret McQuigganFrederick Moore
Oct 11, 2005·Nutrition in Clinical Practice : Official Publication of the American Society for Parenteral and Enteral Nutrition·Alan B MarrFrederick A Moore
Apr 21, 2006·Critical Care Medicine·Ravi S RadhakrishnanCharles S Cox
Mar 24, 2005·World Journal of Gastroenterology : WJG·Ji-Ren YuShu-Sen Zheng
Feb 6, 2017·American Journal of Physiology. Gastrointestinal and Liver Physiology·Viviana FilpaGiovanni Porta
Apr 28, 2018·American Journal of Physiology. Heart and Circulatory Physiology·Asmaa M Almohanna, Susan Wray
Jan 15, 2005·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Hua Tian Gan, J D Z Chen
Feb 12, 2005·The Journal of Trauma·Stacey D Moore-OlufemiCharles S Cox
Dec 29, 2007·Journal of Pediatric Gastroenterology and Nutrition·Hale SayanS Oktay Arslan

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