Inducible nitric oxide synthase response and associated cytokine gene expression in the spleen of mice infected with Clonorchis sinensis

Parasitology Research
Ji-Qing ShenXiao-Nong Zhou

Abstract

Clonorchis sinensis is a food-borne parasite that induces a permanent increase of nitrosation in the body upon infection. The spleen is an important secondary lymphoid organ for the regulation of immune responses locally and in the whole body. However, the functions and mechanisms of the spleen in nitric oxide (NO) responses after C. sinensis infection remain unknown. In this study, BALB/c mice were infected with 20, 40, and 80 C. sinensis metacercariae to simulate mild, moderate, and severe infections, respectively. We examined the expression of inducible nitric oxide synthase (iNOS) in the spleen and the relevant cytokine transcription in splenocytes from the mice infected with different amounts of metacercariae. The iNOS of the mice infected with 80 metacercariae was expressed in the spleen as early as 10 days post-infection (dpi) and gradually increased until 90 dpi. The iNOS expression in the mice infected with 40 metacercariae was detected only at 45 and 90 dpi, but not in the mice infected with 20 metacercariae. The level of interferon (IFN)-γ messenger RNA (mRNA) transcription in splenocytes significantly increased at 10 and 20 dpi (P < 0.05) in response to mild/moderate infection but gradually decreased to normal level...Continue Reading

References

Apr 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·D A GellerT R Billiar
Aug 17, 2000·Immunologic Research·V K SinghS S Agarwal
Dec 9, 2000·The Journal of Biological Chemistry·Kalipada PahanT M Petro
Feb 24, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·H SchindlerC Bogdan
Mar 10, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·L DasP K Das
Aug 23, 2001·International Immunopharmacology·R C van der Veen
Jul 11, 2002·Parasitology Research·Elzbieta Wandurska-Nowak, Justyna Wiśniewska
Aug 3, 2002·The British Journal of Surgery·P Watanapa, W B Watanapa
Aug 5, 2003·Parasitology Research·Yang Kyu ChoiDae Yong Kim
Dec 29, 2004·The Lancet Infectious Diseases·Zhao-Rong LunYue-Yi Fang
Sep 13, 2005·Journal of Helminthology·H-J Rim
Aug 2, 2007·Nature Reviews. Drug Discovery·Csaba SzabóRafael Radi
Sep 26, 2007·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Manel AmriChafia Touil-Boukoffa
Dec 6, 2008·Folia Biologica·Ewa JablonskaJakub Jablonski
Jan 21, 2010·Cancer Science·Hai-Rim ShinSung-Tae Hong
Mar 10, 2011·Cancer Immunology, Immunotherapy : CII·Nejat K EgilmezMehmet O Kilinc
Jul 12, 2011·Parasitology International·Sung-Tae Hong, Yueyi Fang
Jun 27, 2013·World Journal of Gastroenterology : WJG·Giovanni TarantinoCarmine Finelli
Jan 1, 2012·Infectious Diseases of Poverty·Men-Bao QianXiao-Nong Zhou
Aug 21, 2013·Cytokine·Joo-Yun YimChang-Yeol Yim
Oct 12, 2013·Frontiers in Immunology·Charles A DinarelloGilles Kaplanski
Nov 19, 2013·Immunity·Vincenzo Bronte, Mikael J Pittet
Jan 11, 2014·Journal of Hepato-biliary-pancreatic Sciences·Paiboon SithithawornChawalit Pairojkul

Related Concepts

Clonorchis sinensis
Gene Expression
Mice, Inbred BALB C
Laboratory mice
Nitric Oxide
Parasites
RNA, Messenger
Spleen
Transcription, Genetic
Cytokine

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