PMID: 8998998Apr 1, 1996Paper

Inducible production and cellular location of the epidermin biosynthetic enzyme EpiB using an improved staphylococcal expression system

FEMS Microbiology Letters
A PeschelF Götz

Abstract

The antimicrobial peptide epidermin is distinguished by thioether amino acids such as meso-lanthionine, 3-methyl-lanthionine, and 2-aminovinylcysteine. The enzyme EpiB, encoded on a plasmid of the producing strain Staphylococcus epidermidis Tü3298, is very likely involved in the formation of these unusual amino acids. In order to obtain high-level production of EpiB, an improved staphylococcal expression vector based on the xylose-inducible xylA promoter of Staphylococcus xylosus was constructed. As shown by the expression of a lipase reporter gene, the new plasmid pTX15 mediated a considerably higher expression level after induction and a lower background expression level in the uninduced state than the previously described vector pCX15. The epiB gene was inserted in pTX15 and expressed in Staphylococcus carnosus. The EpiB protein was detected both in the cytoplasmic and the membrane fraction and was partially purified in three steps.

References

Nov 1, 1992·Applied and Environmental Microbiology·G EngelkeK D Entian
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Feb 15, 1992·European Journal of Biochemistry·N SchnellK D Entian
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Jul 1, 1993·Molecular Microbiology·A PeschelF Götz
Nov 1, 1995·Applied and Environmental Microbiology·B OttenwälderF Götz

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Citations

Feb 10, 2011·The Journal of Biological Chemistry·Mami NishieKenji Sonomoto
Aug 29, 2012·Antimicrobial Agents and Chemotherapy·Jongkon SaisingFriedrich Götz
Dec 9, 2008·Applied and Environmental Microbiology·Ralf RosensteinFriedrich Götz
Dec 20, 2011·Applied and Environmental Microbiology·Bernhard KrismerAndreas Peschel
Jun 9, 2004·Applied and Environmental Microbiology·Anja HoffmannHans-Georg Sahl
Jul 28, 2009·Journal of Bacteriology·Lalitha BiswasFriedrich Götz
Sep 11, 2007·Journal of Molecular Microbiology and Biotechnology·Fang ChengPer E J Saris
Oct 16, 2010·PLoS Pathogens·Gordon Y C CheungMichael Otto
Oct 15, 2013·International Journal of Medical Microbiology : IJMM·Friedrich GötzMartin Schlag
Jul 20, 2002·Biochemical and Biophysical Research Communications·Lili XieWilfred A van der Donk
Nov 17, 2009·Journal of Bioscience and Bioengineering·Mami NishieKenji Sonomoto
Jan 14, 2009·International Journal of Medical Microbiology : IJMM·Peter Sass, Gabriele Bierbaum
Apr 10, 2007·International Journal of Medical Microbiology : IJMM·Andrea JansenGabriele Bierbaum
Oct 27, 2007·Molecular Microbiology·Min LiMichael Otto
Nov 26, 2010·Molecular Microbiology·Des FieldR Paul Ross
Feb 5, 2013·Microbiology·Marcel PraxRalph Bertram
Oct 14, 2008·Current Opinion in Microbiology·Leif Smith, Jd Hillman
Apr 23, 2016·Nature Protocols·Volker WinstelAndreas Peschel
Jun 1, 2006·FEMS Microbiology Letters·Raja BiswasFriedrich Götz
Apr 21, 2009·Methods in Enzymology·Bo LiWilfred A van der Donk
Dec 15, 2015·Scientific Reports·Gordon Y C CheungMichael Otto
Oct 4, 2017·Applied Microbiology and Biotechnology·John LöfblomFriedrich Götz
Jan 27, 2015·Applied and Environmental Microbiology·Volker WinstelHolger Rohde
Jul 16, 2005·The Journal of Biological Chemistry·Alexandra PelzFriedrich Götz

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