Inducible tyrosine phosphorylation of the beta3 integrin requires the alphaV integrin cytoplasmic tail.

The Journal of Biological Chemistry
Scott D BlystoneE J Brown

Abstract

We have found that the integrin beta3 chain can be phosphorylated on tyrosine residues in K562 cells transfected with alphavbeta3. Tyrosine phosphorylation of the beta3 cytoplasmic tail is induced by adhesion to alphavbeta3-specific ligand or antibody or by incubation in manganese-containing buffer. Under the same conditions, beta5 does not become tyrosine-phosphorylated in K562 transfected with alphavbeta5. Phosphorylation of the beta3 subunit requires the simultaneous presence of the alphav subunit cytoplasmic tail, because neither the alphaIIb subunit nor a truncated alphav subunit is sufficient to permit phosphorylation of beta3 when coexpressed as a heterodimer with beta3. Finally, tyrosine phosphorylation of the beta3 cytoplasmic tail occurs on both human and murine beta3 and is inducible in the ovarian carcinoma OV10 as well, independent of expression of integrin-associated protein (CD47). Tyrosine phosphorylation of the beta3 integrin subunit facilitates association of Grb-2, an adaptor protein leading to activation of the Ras signaling pathway, and may contribute to the unique functional and signaling capabilities of alphavbeta3.

References

Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Mar 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·R E SeftorM J Hendrix
Jun 1, 1992·The Journal of Clinical Investigation·B Felding-HabermannD A Cheresh
Sep 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·R HirstL Rohrschneider
Dec 2, 1994·Science·K Vuori, E Ruoslahti
Jun 23, 1995·The Journal of Biological Chemistry·Z SongyangL C Cantley
Sep 22, 1995·The Journal of Biological Chemistry·S A PrigentW J Gullick
Apr 14, 1995·The Journal of Biological Chemistry·T E O'TooleB M Culley
Apr 25, 1995·Proceedings of the National Academy of Sciences of the United States of America·D CooperM A Vadas
Jan 5, 1996·The Journal of Biological Chemistry·A G GaoW A Frazier

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Citations

Oct 26, 1999·Cellular Signalling·G Berton, C A Lowell
Dec 24, 1997·Matrix Biology : Journal of the International Society for Matrix Biology·S E LaFlammeA M Mastrangelo
Dec 24, 1997·Matrix Biology : Journal of the International Society for Matrix Biology·T E O'Toole
Sep 7, 2001·Current Opinion in Cell Biology·D R PhillipsL Nannizzi-Alaimo
Sep 5, 1998·Trends in Cell Biology·P E Hughes, M Pfaff
Feb 25, 2000·The International Journal of Biochemistry & Cell Biology·M G Coppolino, S Dedhar
Jul 13, 2000·Molecular and Cellular Biology·K WennerbergS Johansson
Apr 16, 2009·Journal of Cell Science·Ramona BhattacharyaJonathan C R Jones
Mar 22, 2001·Proceedings of the National Academy of Sciences of the United States of America·T SakaiD F Mosher
Oct 14, 2004·The Journal of Cell Biology·DeannaLee M BeauvaisAlan C Rapraeger
Nov 21, 1998·Cell Adhesion and Communication·Y van KooykC G Figdor
Nov 12, 2009·Journal of Virological Methods·Neelanjana RayAndrea Bertolotti-Ciarlet
Nov 28, 2001·The Journal of Biological Chemistry·Anirban DattaDavid Boettiger
Jun 6, 1997·The Journal of Biological Chemistry·J ChungW A Frazier
Jul 27, 2001·The Journal of Biological Chemistry·M LeeJ E Schwarzbauer
Oct 3, 2002·The Journal of Biological Chemistry·Scott D Blystone
Jul 5, 2018·Cell Communication and Signaling : CCS·Zhongyuan CaoYan-Qing Ma
Nov 26, 2002·The Journal of Biological Chemistry·Boyd ButlerScott D Blystone
Jan 2, 2009·Journal of Cell Science·Kyle R Legate, Reinhard Fässler
Mar 10, 2001·Journal of Vascular Surgery·M SobelY Suda
Feb 6, 2007·European Journal of Cell Biology·Francis FouchierSerge Champion

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