Induction and accumulation of polyphenol oxidase activities as implicated in development of resistance against pearl millet downy mildew disease

Functional Plant Biology : FPB
S Niranjan RajH Shekar Shetty

Abstract

Polyphenol oxidase (PPO) activity was analysed in seedlings of resistant and susceptible pearl millet [Pennisetum glaucum (L.) R.Br] cultivars with or without inoculation of the downy mildew pathogen Sclerospora graminicola (Sacc.) Schroet. Seedlings of resistant varieties had greater PPO activity than susceptible seedlings, and inoculated seedlings had significantly higher PPO levels than uninoculated seedlings. Temporal accumulation of PPO showed a maximum activity at 24 h post-inoculation in resistant seedlings, whereas in susceptible seedlings it peaked at 48 h. PPO activity was positively correlated with levels of downy mildew resistance in different pearl millet cultivars under field conditions. Native PAGE staining showed four isoforms of PPO, which were differentially induced in relation to the time of appearance and intensities in the uninoculated seedlings, whereas a fifth PPO isoform appeared after inoculation with S. graminicola. PPO activity was significantly higher in the shoot and leaves of pearl millet than in the root. Tissue printing analysis of the enzyme expression showed that the enzyme is predominantly expressed after pathogen inoculation and is localised in the epidermal and vascular regions. Temporal ana...Continue Reading

Citations

Sep 14, 2007·Molecular Plant-microbe Interactions : MPMI·H Peter van EsseBart P H J Thomma
Mar 14, 2012·Acta Biochimica Et Biophysica Sinica·S Ashok PrabhuH S Shetty
Oct 30, 2013·Molecular Biology Reports·Pushpalatha Hosur GnanaprakashShekar Hunthrike Shetty
Jul 21, 2012·Plant Molecular Biology·Mareike E Dirks-HofmeisterBruno M Moerschbacher
Feb 18, 2017·International Journal of Molecular Sciences·Francesca TarantoCinzia Montemurro
Jun 17, 2008·Applied and Environmental Microbiology·Kathryn L OliverWilliam E Hintz
Mar 16, 2013·Critical Reviews in Microbiology·Surinder Kaur, Gurpreet Singh Dhillon
Nov 25, 2020·Phytochemistry·Jin Zhang, Xiaoling Sun
Apr 2, 2021·Journal of the Science of Food and Agriculture·Shan Ahamed TharifkhanChinnaswamy Anandharamakrishnan
Jan 19, 2010·Journal of Agricultural and Food Chemistry·Ifat ParveenAna Winters
Jun 25, 2013·Journal of Agricultural and Food Chemistry·K Judith WebbAna L Winters

❮ Previous
Next ❯

Methods Mentioned

BETA
transgenic
electrophoresis

Software Mentioned

RS Image Analysis
Bioprofile Image Analysis system
JMP

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

© 2021 Meta ULC. All rights reserved