PMID: 8939793Jan 1, 1997Paper

Induction and Characterization of Laccase in the Ligninolytic Fungus Pleurotus eryngii

Current Microbiology
C MunozM J Martinez

Abstract

Two protein bands with laccase activity were found after PAGE of culture liquid or mycelium extract of Pleurotus eryngii, grown on glucose-ammonium tartrate-yeast extract medium with and without inducers. A major and a minor laccase band were observed in the basal medium. The intensity of the major band (laccase I) did not change after the addition of inducers. However, the minor band (laccase II), characterized by higher electrophoretic mobility, was strongly induced by wheat-straw alkalilignin and vanillic and veratric acids. Laccase activity in the basal medium had an optimum pH of 4.5 and was stable from pH 3 to 10 during 24 h at room temperature. This enzyme had wide substrate specificity on hydroquinones, methoxy-substituted monophenols, and aromatic amines. In general, laccase activity was found only with compounds having a redox potential lower than 0.5 mV. The highest activity was obtained with methoxy- and methyl-substituted p-hydroquinones and aromatic diamines. Some activity also occurred with the aliphatic compound 3,5-cyclohexadiene-1,2-diol.

Citations

Nov 30, 2000·Archives of Biochemistry and Biophysics·F GuillénA T Martínez
Jan 14, 2005·Applied Microbiology and Biotechnology·René UllrichMartin Hofrichter
Apr 26, 2006·Applied Microbiology and Biotechnology·H X Wang, T B Ng
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