Induction and long-term maintenance of Thy-1 positive T lymphocytes: derivation from continuous bone marrow cultures

Journal of Supramolecular Structure
G TertianM A Moore

Abstract

In the present study we investigated the presence of T-lymphocyte progenitors in the long-term murine bone marrow culture system described by Dexter: mature Thy-1 antigen-bearing T lymphocytes are lost in these cultures after a few days. By culturing nonadherent cells from such cultures in the presence of a supernatant of concanavalin A-stimulated spleen cells, a source of T-cell growth factor, we found that Thy-1 positive blast cells proliferated together with a second population of Thy-1 negative cells. These two populations of cells have been maintained in long-term in vitro cultures by passaging the cells in fresh conditioned medium at regular intervals. Moreover, we have been able to establish pure cultures of the Thy-1 bearing blast cells after separating them from the non-T cells using their adherence property to plastic surfaces. Long-term cultures of T lymphocytes can thus be established from long-term marrow cultures as well as from the spleen, thymus or fresh bone marrow.

References

Jul 14, 1977·Nature·S Gillis, K A Smith
Jun 1, 1979·The Journal of Experimental Medicine·S GillisK A Smith
Jun 1, 1977·Journal of Cellular Physiology·T M DexterL G Lajtha
Oct 1, 1978·The Journal of Experimental Medicine·S GillisK A Smith
Jan 1, 1979·The Journal of Experimental Medicine·P E BakerK A Smith
Sep 14, 1978·Nature·T M Dexter, E Spooncer
Jan 1, 1977·Cold Spring Harbor Symposia on Quantitative Biology·P W Kincade, P Ralph
Dec 1, 1974·British Journal of Haematology·T M Dexter, L G Lajtha
Dec 1, 1973·Journal of Cellular Physiology·T M DexterB I Lord

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Citations

Apr 15, 1985·Klinische Wochenschrift·H G Mergenthaler
Sep 1, 1986·International Journal of Cell Cloning·E S StrobelP L Greenberg

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