Induction and loss of Ti plasmid conjugative competence in response to the acyl-homoserine lactone quorum-sensing signal.

Journal of Bacteriology
Shengchang SuS K Farrand

Abstract

Conjugative transfer of the Ti plasmids of Agrobacterium tumefaciens is controlled by a quorum-sensing system composed of TraR and its signal N-(3-oxo-octanoyl)-L-homoserine lactone. This system is, in turn, controlled by the conjugative opines produced by crown gall tumors induced on plants by the bacteria. Using nonpolar traI mutants, we examined the kinetics of induction of conjugative transfer in response to exogenous acyl-homoserine lactone. In the absence of the antiactivator TraM, onset of induction of transfer requires about 30 min, 15 to 20 min of which is needed for expression and construction of the conjugative apparatus. TraM delays the onset of conjugation by 30 min. While the rate of development of conjugative competence was not significantly affected by levels of TraR, maximum efficiencies of transfer were correlated with amounts of the activator in the donors. Donors harboring Ti plasmids lacking TraM were fully induced by the quormone at concentrations as low as 100 pM. TraM raised the concentration of signal required for maximum activity to 1 nM. Donors grown in batch culture retained conjugative competence following signal removal, even when in stationary phase. However, donors kept in balanced growth rapidly...Continue Reading

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Citations

Jul 5, 2008·Proceedings of the National Academy of Sciences of the United States of America·Yancheng LiuZhao-Qing Luo
Jul 11, 2012·Proceedings of the National Academy of Sciences of the United States of America·Hidetada HirakawaE Peter Greenberg
Apr 10, 2010·Proceedings of the National Academy of Sciences of the United States of America·Richard SiehnelPradeep K Singh
Sep 24, 2013·Journal of Bacteriology·John H Kimbrough, Eric V Stabb
Oct 10, 2012·Molecular Microbiology·Sara PlanamenteDenis Faure
Feb 9, 2012·Mobile Genetic Elements·Catherine Guynet, Fernando de la Cruz
Jan 6, 2017·Scientific Reports·Mélanie TannièresDenis Faure
Dec 24, 2013·Journal of Bacteriology·Margaret E WetzelStephen K Farrand
Jan 28, 2021·Microorganisms·Julie BaltenneckFlorence Hommais
Jan 17, 2015·Microbiology Spectrum·Jay E Gordon, Peter J Christie

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