INDUCTION AND PROPERTIES OF A TEMPERATURE BACTERIOPHAGE FROM BACILLUS STEAROTHERMOPHILUS.

Journal of Bacteriology
N E WELKER, L L Campbell

Abstract

Welker, N. E. (University of Illinois, Urbana), and L. Leon Campbell. Induction and properties of a temperate bacteriophage from Bacillus stearothermophilus. J. Bacteriol. 89:175-184. 1965.-Bacillus stearothermophilus 1503-4R growing at 55 C was induced to lyse either when 0.05 mug/ml of mitomycin C was added or when it was exposed to ultraviolet light for 30 sec. Lysis of the induced cultures occurred 45 to 60 min after induction. Phage were assayed on B. stearothermophilus 4S giving turbid plaques 0.05 to 0.3 cm in diameter. Noninduced cultures of 1503-4R spontaneously produced one phage per 2.8 x 10(6) bacterial cells. The optimal temperature for phage production and assay was found to be 55 C. B. stearothermophilus 1503-4R was immune to the isolated temperate phage (TP-1) and to a clear-plaque mutant phage (TP-1C), even when tested at phage mutliplicities of 100. TP-1 and TP-1C phage were identical morphologically having a head 65 mmu in diameter and a tail 240 mmu long and 12 mmu wide. TP-1C phage deoxyribonucleic acid (DNA) had an S(20,w) value of 24.1 and a calculated molecular weight of 1.21 x 10(7). DNA base compositions of TP-1 and TP-1C phage were identical (42% guanine + cytosine), but differed significantly from th...Continue Reading

References

Aug 31, 1962·Biochemical and Biophysical Research Communications·R BEN-GURION

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Citations

Oct 1, 1966·Canadian Journal of Microbiology·L Blouse, R Twarog
Mar 1, 1968·The Journal of Applied Bacteriology·L L Campbell, B Pace
Jan 1, 1969·Archiv für Mikrobiologie·J J Patel
Jun 1, 1967·Journal of Virology·L N Egbert, H K Mitchell
Jun 1, 1971·Journal of Bacteriology·U N Streips, F E Young
Jun 1, 1971·Journal of Bacteriology·U N Streips, N E Welker
Jul 1, 1969·Journal of Bacteriology·U N Streips, N E Welker
Apr 1, 1967·Journal of Bacteriology·A B Sutow, N E Welker
Feb 1, 1975·Applied Microbiology·I Epstein, L L Campbell
Apr 1, 1973·Journal of Virology·N D Lees, N E Welker
Oct 1, 1967·Journal of Bacteriology·N E Welker, L L Campbell
Feb 1, 1977·Journal of Bacteriology·J F Catterall, N E Welker
Jan 1, 1966·Journal of Bacteriology·G F Saunders, L L Campbell
Jan 1, 1966·Journal of Bacteriology·G F Saunders, L L Campbell

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