Induction and regulation of interleukin-6 gene expression in rat astrocytes

Journal of Neuroimmunology
E N BenvenisteG M Fuller

Abstract

Cells that produce interleukin-6 (IL-6) require the presence of signaling molecules since this cytokine is not normally constitutively expressed. It is now established that astrocytes produce IL-6; however, the precise inducing molecules and the kinetics of their action have not yet been clearly identified. In the current study, we show that either interleukin-1 beta (IL-1 beta) or tumor necrosis factor-alpha (TNF-alpha) exert a strong inducing signal for IL-6 in primary rat astrocytes. When the two cytokines are added together the response is synergistic, suggesting that each cytokine may induce IL-6 gene expression by different pathways. Interferon-gamma (IFN-gamma) does not affect IL-6 expression although if it is added in conjunction with IL-1 beta, an augmented induction of IL-6 occurs. In addition to the cytokines, bacterial lipopolysaccharide (LPS) and the calcium ionophore, A23187, induce IL-6 expression. IL-6 expression can be blocked by the glucocorticoid analogue, dexamethasone. IL-6 induction by LPS/Ca2+ ionophore is more sensitive to the suppressive effects of dexamethasone than is IL-6 induction by TNF-alpha/IL-1 beta. Cycloheximide (CHX), an inhibitor of protein synthesis, markedly increased levels of IL-6 mRNA i...Continue Reading

References

Dec 1, 1977·Analytical Biochemistry·S W KwanR M Goldblum
Aug 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·A P LiebermanM L Shin
Jul 1, 1989·Cellular Immunology·M R ShalabyT Espevik
Nov 1, 1989·The Journal of Experimental Medicine·M E Lanser, G E Brown
Aug 1, 1989·The Journal of Experimental Medicine·F M HofmanJ E Merrill
May 1, 1989·The Journal of Clinical Investigation·G J FreemanL M Nadler
Aug 1, 1989·European Journal of Immunology·M RighiP Ricciardi-Castagnoli
Sep 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·Y H ZhangJ Vilcek
Dec 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·J Van SnickR J Simpson
Nov 29, 1985·Science·B M WoloskiJ E Blalock
May 1, 1988·Immunology Today·G G Wong, S C Clark
Jun 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·P B SehgalI Tamm
Sep 1, 1987·European Journal of Immunology·K FreiA Fontana
Apr 1, 1988·Journal of the Neurological Sciences·U Traugott, P Lebon
Feb 1, 1988·Immunology Today·A O'GarraJ Christiansen
Jun 30, 1988·Biochemical and Biophysical Research Communications·K NoriokaH Nakamura
Oct 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·C P ChiuF Lee
May 1, 1988·DNA·P E DanielsonJ G Sutcliffe
Aug 1, 1986·The Journal of Experimental Medicine·D GiulianL B Lachman
Oct 1, 1987·European Journal of Immunology·L A AardenP M Lansdorp
Dec 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·J WeissenbachM Revel
Jun 1, 1980·The Journal of Cell Biology·K D McCarthy, J de Vellis

❮ Previous
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Citations

Dec 24, 1997·Clinical Immunology and Immunopathology·A DiabM Bakhiet
Jan 1, 1992·Neurosurgical Review·T NittaL Steinman
Jan 1, 1996·Springer Seminars in Immunopathology·K Selmaj
Feb 11, 1998·Molecular Neurobiology·D L Gruol, T E Nelson
Mar 26, 2009·Cellular and Molecular Neurobiology·Andreas Hald
Dec 1, 1992·Journal of Neuroimmunology·J A MartineyC F Brosnan
Jul 1, 1992·Journal of Neuroimmunology·M C Morganti-KossmannS M Wahl
Nov 1, 1994·Journal of Neuroimmunology·C Brodie, N Goldreich
Jan 1, 1996·Journal of Neuroimmunology·H E de VriesJ Kuiper
May 1, 1996·Brain Research. Molecular Brain Research·F PoussetP Soubrie
Jun 1, 1995·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·I L Campbell
Jul 1, 1994·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·G SchettiniP Preziosi
Mar 21, 1998·Pharmacology & Therapeutics·F Zavala
Mar 1, 1997·Journal of Neuroimmunology·S M LeVine, D C Brown
Aug 1, 1997·Progress in Neurobiology·R A Gadient, U H Otten

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