PMID: 7515948Jul 1, 1994Paper

Induction and regulation of nitric oxide synthase in retinal Müller glial cells

Journal of Neurochemistry
O GoureauY de Kozak

Abstract

Müller glial cells from the rat retina were examined for their capacity to produce nitric oxide (NO). Treatment of retinal Müller glial (RMG) cells with lipopolysaccharide (LPS), interferon-gamma, and tumor necrosis factor-alpha induced NO synthesis as determined by nitrite release in media. Simultaneous addition of LPS, interferon-gamma, and tumor necrosis factor-alpha caused the largest increase in NO synthesis. NO biosynthesis was detected after 12 h and was dependent on the dose of LPS, interferon-gamma, and tumor necrosis factor-alpha. Stereoselective inhibitors of NO synthase (NOS), cycloheximide and transforming growth factor-beta, blocked cytokine-induced NO production. Cytosol from LPS/cytokine-treated RMG cultures, but not from unstimulated cultures, produced a calcium/calmodulin-independent conversion of L-arginine to L-citrulline that was completely blocked by NOS inhibitor. The expression of NOS in RMG cells was confirmed by northern blot analysis, in which stimulation of these cells led to an increase in NOS mRNA levels. We conclude that RMG cells can express an inducible form of NOS similar to the macrophage isoform. High NO release from activated RMG cells might represent a protection from infection but may also...Continue Reading

Citations

Aug 16, 2002·Molecular Pharmacology·Akira AndoPeter A Campochiaro
Sep 28, 2005·Japanese Journal of Ophthalmology·Ryotaro GotoYukitaka Uji
Jan 1, 1994·Journal of Molecular Neuroscience : MN·J R Sparrow
Aug 1, 1996·Cellular and Molecular Neurobiology·C S ParkG Krishna
Sep 1, 1995·Journal of Neuroscience Research·D H AndersonK C Flanders
Jun 11, 2002·Retina·Gürsel YilmazPinar Aydin
Apr 19, 2007·Ophthalmologica. Journal international d'ophtalmologie. International journal of ophthalmology. Zeitschrift für Augenheilkunde·Turgut YilmazBilal Ustundağ
Jun 26, 2002·Clinical Genetics·G KumaramanickavelT Sharma
Mar 23, 2011·British Journal of Pharmacology·Melanie J MurphySheila G Crewther
Nov 1, 1995·The European Journal of Neuroscience·K R Huxlin, M R Bennett
Nov 8, 2001·American Journal of Physiology. Cell Physiology·C C BridgesS B Smith
Aug 27, 1999·Journal of Ocular Pharmacology and Therapeutics : the Official Journal of the Association for Ocular Pharmacology and Therapeutics·S HarinoP Reinach
Jan 5, 2006·Free Radical Research·Mutay AslanSalih Sanlioglu
Apr 7, 2017·Journal of Neuroinflammation·Subramanian DharmarajanTeri L Belecky-Adams
Aug 5, 2005·Visual Neuroscience·Alejandro BerraLeonor Sterin-Borda
Oct 23, 1997·Journal of Neuroscience Research·O GoureauF Chany-Fournier
Apr 13, 1999·Biochemical and Biophysical Research Communications·S B SmithV Ganapathy
Sep 1, 1996·Vision Research·I M GoldsteinS Roth
Jul 1, 1997·Survey of Ophthalmology·F BecquetO Goureau
Oct 23, 1995·The Journal of Comparative Neurology·D E KurenniS Barnes
May 2, 2008·Progress in Retinal and Eye Research·Constantin J PournarasEinar Stefansson
Aug 8, 2009·Progress in Retinal and Eye Research·Andreas BringmannAndreas Reichenbach

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