Abstract
The comet assay is widely used in human biomonitoring to measure DNA damage in whole blood or isolated peripheral blood mononuclear cells (PBMC) as a marker of exposure to genotoxic agents. Cytogenetic assays with phytohemagglutinin (PHA)-stimulated cultured T lymphocytes are also frequently performed in human biomonitoring. Cytogenetic effects (micronuclei, chromosome aberrations, sister chromatid exchanges) may be induced in vivo but also occur ex vivo during the cultivation of lymphocytes as a consequence of DNA damage present in lymphocytes at the time of sampling. To better understand whether DNA damage measured by the comet assay in PBMC is representative for DNA damage in T cells, we comparatively investigated DNA damage and its repair in PBMC and T cells obtained by immunomagnetic cell sorting. PBMC cultures and T cell cultures were exposed to mutagens with different modes of genotoxic action and DNA damage was measured by the comet assay after the end of a 2h exposure and after 18h post-incubation. The mutagens tested were methyl methanesulfonate (MMS), (±)-anti-B[a]P-7,8-dihydrodiol-9,10-epoxide (BPDE), 4-nitroquinoline-1-oxide (4NQO), styrene oxide and potassium bromate. MMS and potassium bromate were also tested by ...Continue Reading
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Citations
Sep 9, 2015·Mutagenesis·Julia BausingerGünter Speit
May 24, 2015·Mutagenesis·Julia Bausinger, Günter Speit
Feb 21, 2019·Environmental and Molecular Mutagenesis·Martha M MooreTamara House-Knight
Jun 10, 2017·AIMS Genetics·Michael T Fasullo, Mingzeng Sun
Apr 23, 2020·Mutagenesis·Peter MøllerAndrew Collins
May 8, 2016·Mutagenesis·Julia Bausinger, Günter Speit
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