Induction and repair of DNA damage measured by the comet assay in human T lymphocytes separated by immunomagnetic cell sorting

Mutation Research
Julia Bausinger, Günter Speit

Abstract

The comet assay is widely used in human biomonitoring to measure DNA damage in whole blood or isolated peripheral blood mononuclear cells (PBMC) as a marker of exposure to genotoxic agents. Cytogenetic assays with phytohemagglutinin (PHA)-stimulated cultured T lymphocytes are also frequently performed in human biomonitoring. Cytogenetic effects (micronuclei, chromosome aberrations, sister chromatid exchanges) may be induced in vivo but also occur ex vivo during the cultivation of lymphocytes as a consequence of DNA damage present in lymphocytes at the time of sampling. To better understand whether DNA damage measured by the comet assay in PBMC is representative for DNA damage in T cells, we comparatively investigated DNA damage and its repair in PBMC and T cells obtained by immunomagnetic cell sorting. PBMC cultures and T cell cultures were exposed to mutagens with different modes of genotoxic action and DNA damage was measured by the comet assay after the end of a 2h exposure and after 18h post-incubation. The mutagens tested were methyl methanesulfonate (MMS), (±)-anti-B[a]P-7,8-dihydrodiol-9,10-epoxide (BPDE), 4-nitroquinoline-1-oxide (4NQO), styrene oxide and potassium bromate. MMS and potassium bromate were also tested by ...Continue Reading

References

Dec 3, 2003·Toxicology Letters·Günter SpeitHeike Hoffmann
Dec 12, 2003·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·J M PoulP Sanders
Sep 30, 2004·Toxicology Letters·Günter SpeitHeike Hoffmann
Oct 7, 2004·Environmental and Molecular Mutagenesis·Lode GodderisDominique Lison
Feb 20, 2008·Mutagenesis·Andrew R CollinsRudolf Stetina
Dec 6, 2011·Toxicology in Vitro : an International Journal Published in Association with BIBRA·So-Young ParkDonggeun Sul
Nov 5, 2013·Mutation Research. Reviews in Mutation Research·Andrew CollinsUNKNOWN ComNet project

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Citations

Apr 23, 2020·Mutagenesis·Peter MøllerAndrew Collins
Oct 11, 2017·Mutagenesis·Peter MøllerSteffen Loft
Sep 27, 2019·Journal of Psychopharmacology·Mahnaz AhmadimaneshLeila Etemad

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