PMID: 6975250Oct 1, 1981Paper

Induction and repair of single-strand DNA breaks after X-irradiation of human fibroblasts deficient in glutathione

International Journal of Radiation Biology and Related Studies in Physics, Chemistry, and Medicine
M R EdgrenA Larsson

Abstract

Using the unwinding technique in weak alkali, the induction and repair of DNA single-strand breaks was determined after aerobic and anerobic X-irradiation of human fibroblasts, obtained from a patient suffering from 5-oxoprolinuria, and from a clinically healthy control. The metabolic disorder associated with 5-oxprolinuria is a deficiency in glutathione synthetase activity resulting in a greatly reduced glutathione content in the cells. A small dose-modifying effect of oxygen (o.e.r. = 1.1) was found for these cells in comparison to an o.e.r. of 2.5 for control cells with normal glutathione content. No significant difference was found between the repair capacity of cells with normal and deficient glutathione content, and repair was nearly completed within 60 min of anoxic irradiation in each case. In contrast, after aerobic irradiation of glutathione-deficient cells repaired less than 70 per cent of the breaks during the same period. When the glutathione-deficient cells were incubated with either dithiothreitol or mercaptopropionylglycine directly after aerobic irradiation, almost complete repair was obtained within 60 Min. The data are interpreted as indicating that the repair mechanism for oxically and anoxically induced sin...Continue Reading

References

Jul 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·A Holmgren
Nov 15, 1976·Clinica Chimica Acta; International Journal of Clinical Chemistry·A LarssonU Porath
May 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·M LuthmanL Thelander
Jun 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·V P WellnerA Larsson
Feb 25, 1971·Biochimica Et Biophysica Acta·M G Ormerod, U Stevens
Mar 27, 1954·Nature·P ALEXANDER, A CHARLESBY
May 1, 1959·Archives of Biochemistry and Biophysics·G L ELLMAN

❮ Previous
Next ❯

Citations

Feb 23, 2013·Archives of Environmental & Occupational Health·Anabela MiraDaniel M López-Larraza
Mar 1, 1983·The British Journal of Radiology·B Mukherjee
Jan 1, 1995·Acta Oncologica·K Rosander, B Zackrisson
Jul 1, 1987·International Journal of Radiation Biology and Related Studies in Physics, Chemistry, and Medicine·S W Dean
Aug 1, 1987·International Journal of Radiation Biology and Related Studies in Physics, Chemistry, and Medicine·D FrankenbergF Eckardt-Schupp
Jul 1, 1986·International Journal of Radiation Biology and Related Studies in Physics, Chemistry, and Medicine·O VosW S Roos-Verheij
Jan 1, 1987·International Journal of Radiation Biology and Related Studies in Physics, Chemistry, and Medicine·G SölenL Révész
Aug 1, 1985·International Journal of Radiation Biology and Related Studies in Physics, Chemistry, and Medicine·M Edgren, L Révész
Oct 1, 1985·International Journal of Radiation Biology and Related Studies in Physics, Chemistry, and Medicine·D DebieuE P Malaise
Jan 1, 1986·International Journal of Radiation Biology and Related Studies in Physics, Chemistry, and Medicine·P J DeschavanneL Révész
Apr 1, 1986·International Journal of Radiation Biology and Related Studies in Physics, Chemistry, and Medicine·I R Radford
Jan 1, 1996·Cancer Investigation·C P SchröderR F Ozols
Jul 1, 1986·International Journal of Radiation Oncology, Biology, Physics·M R EdgrenL Révész
Aug 1, 1984·International Journal of Radiation Oncology, Biology, Physics·O VosW S Roos-Verhey
May 15, 1994·International Journal of Radiation Oncology, Biology, Physics·L RévészA A Wainson
Apr 1, 1989·Radiotherapy and Oncology : Journal of the European Society for Therapeutic Radiology and Oncology·M Frankenberg-Schwager
Jan 1, 1983·Radiation and Environmental Biophysics·G SimoneM Quintiliani
Jun 8, 2006·Environmental and Molecular Mutagenesis·Ganesh C JagetiaManjeshwar S Baliga
Jan 1, 1990·Radiation and Environmental Biophysics·M Frankenberg-Schwager
Dec 14, 1999·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·R Jorquera, R M Tanguay
Mar 1, 1996·International Journal of Radiation Biology·D MurrayE Rosenberg
Mar 6, 1997·Biochemical and Biophysical Research Communications·R Jorquera, R M Tanguay
Nov 30, 1983·Clinica Chimica Acta; International Journal of Clinical Chemistry·A LarssonP Moldéus
Nov 1, 1986·Journal of Bacteriology·J T Greenberg, B Demple
Jul 1, 1986·International Journal of Radiation Oncology, Biology, Physics·T J KinsellaA J Fornace

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

International Journal of Radiation Biology and Related Studies in Physics, Chemistry, and Medicine
M R EdgrenO C Scott
International Journal of Radiation Biology and Related Studies in Physics, Chemistry, and Medicine
L Révész
International Journal of Radiation Biology
G SölenL Révész
© 2021 Meta ULC. All rights reserved