PMID: 9439651Jan 24, 1998Paper

Induction by DNA immunization of a protective antitumor cytotoxic T lymphocyte response against a minimal-epitope-expressing tumor

Cancer Immunology, Immunotherapy : CII
A Iwasaki, B H Barber

Abstract

In order to examine the use of DNA immunization to block tumor growth, we have developed a model system in which a defined 9-amino-acid epitope from the nucleoprotein of influenza virus is used as a surrogate tumor-associated antigen. A mastocytoma cell line of DBA/2 origin (P815) was transfected with a plasmid encoding the minimal H-2Kd-restricted NP(147-155) cytotoxic T lymphocyte (CTL) epitope, pCMV/NPep, to generate the cell line designated P815-NPep. Mice primed and boosted once with a plasmid encoding the full-length NP gene, pCMV/NP, but not with the minigene pCMV/NPep, developed a strong NP(147-155)-specific CTL response within 2 weeks after the boost. When challenged with 10(4) P815-NPep cells, pCMV/NP-immunized DBA/2 mice were protected from tumor challenge, whereas control mice immunized with the vector backbone rapidly developed lethal tumor. Importantly, the P815-NPep-immune mice were also protected from a subsequent challenge with the untransfected parental tumor P815. By depleting the NP-immune mice of either CD4+ or CD8+ T cells and then challenging with 10(4) P815-NPep tumor cells, it was determined that the CD8-depleted mice rapidly developed tumors, whereas the CD4-depleted or non-treated mice were protected....Continue Reading

Citations

Sep 5, 2002·International Journal of Cancer. Journal International Du Cancer·Eleni PetridouDimitrios Trichopoulos
Oct 16, 2001·Drugs & Aging·S Matzku, M Zöller
Jul 5, 2005·Expert Opinion on Investigational Drugs·L L An, A Sette
Oct 26, 1999·Immunobiology·M Zöller, S Matzku
Jul 6, 2004·Immunological Reviews·Jens A LeifertJ Lindsay Whitton
Mar 22, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·A Prévost-BlondelH Pircher

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