PMID: 7247391Mar 1, 1981

Induction by glucose of an antimycin-insensitive, azide-sensitive respiration in the yeast Kluyveromyces lactis

Antonie van Leeuwenhoek
I FerreroA Goffeau

Abstract

Increasing the glucose concentration from 0.1 to 10% in exponentially growing cultures of Kluyveromyces lactis CBS 2359 does not repress the antimycin-sensitive respiration (QO2 of 80 microliter O2 . h-1 . mg-1 dry weight) but raises the antimycin-insensitive respiration from 3 to 12 microliter O2 . h-2 . mg-1 dry weight. Antimycin A inhibits the growth of K. lactis on a variety of substrates with the exception of glucose at concentrations equal to or higher than 1% where substantial antimycin-insensitive respiratory rates are induced. It can be concluded that a minimal antimycin-insensitive QO2 is necessary for cellular growth when the normal respiratory pathway is not functional. The antimycin-insensitive respiration elicited by growth in high glucose concentrations is poorly inhibited by hydroxamate and is inhibited by 50% by 90 microM azide or 1 mM cyanide. These concentrations are much higher than those necessary to inhibit cytochrome c oxidase which is not involved in the antimycin-insensitive respiration as was demonstrated by spectral measurements. A pigment absorbing at 555 nm is specifically reduced after addition of glucose to antimycin-inhibited cells. The same pigment is reoxidized by further addition of high conce...Continue Reading

References

Mar 1, 1978·Journal of Bacteriology·D L Edwards, B W Unger
Jan 30, 1978·Biochemical and Biophysical Research Communications·I FerreroP P Puglisi
Dec 1, 1971·Journal of Bacteriology·A M Lambowitz, C W Slayman
Jul 17, 1973·Biochemical and Biophysical Research Communications·A BrunnerJ R Mattoon
Oct 1, 1969·Journal of Bacteriology·A M MacQuillan
Jan 1, 1964·Antonie van Leeuwenhoek·C J BULDER

Citations

Dec 1, 1983·Antonie van Leeuwenhoek·A M ViolaI Ferrero
Jan 1, 1985·Antonie van Leeuwenhoek·T LodiI Ferrero
Jan 7, 2009·Antonie van Leeuwenhoek·Miroslav ZivićB Zivanović
Oct 1, 1982·European Journal of Biochemistry·G Baldacci, E Zennaro
May 15, 2007·Canadian Journal of Microbiology·Claudia BarberioEnrico Casalone
Aug 4, 2009·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Yuriy V PynyahaAndriy A Sibirny
Jan 1, 1986·Antonie van Leeuwenhoek·A M ViolaI Ferrero
May 18, 2010·Molecular Genetics and Metabolism·Helen A L TuppenRobert W Taylor
Sep 13, 2005·Annals of the New York Academy of Sciences·Joanna ZakrzewskaMiroslav Zivić
May 24, 2008·American Journal of Human Genetics·Valeria MassaMassimo Zeviani
May 19, 2004·Biochemical and Biophysical Research Communications·Francesca FarinaClaudio Palleschi
Apr 7, 2015·Biochimica Et Biophysica Acta·Raffaele NicastroPaola Coccetti
Dec 10, 2014·Applied Microbiology and Biotechnology·Christian LöserThomas Bley
Apr 12, 2003·FEMS Yeast Research·Alexandra VeigaMaria C Loureiro-Dias

Related Concepts

Antimycin A1
Cochliobolus
Azides
Endomycopsis
Yeast Proteins
Anhydrous Dextrose
Oxygen Consumption
Cyanogran
Sodium Azide
Carbohydrate Metabolism

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