Abstract
Mesenchymal stem cells (MSCs) with the ability to differentiate into insulin‑producing cells (IPCs) have become the most promising means of therapy for diabetes mellitus. Adipose‑derived stromal cells (AdSCs), having similar characteristics to those of derived MSCs, are known to exhibit extensive proliferation potential and are able to undergo multi‑lineage differentiation. Whether AdSCs can differentiate into insulin‑producing cells (IPCs), however, has not been sufficiently elucidated. Therefore, the present study sought to investigate the in vitro differentiation of rabbit (r)AdSCs into IPCs, which may provide an abundant source of cells to treat diabetes. rADSCs were obtained from liposuction aspirates and then induced with glucagon‑like peptide‑1 and nicotinamide to differentiate into insulin‑secreting cells. Differentiation was evaluated by the analysis of morphology, dithizone (DTZ) staining, reverse transcription polymerase chain reaction (RT‑PCR), western blot analysis and a glucose challenge assay with detection of insulin secretion by ELISA. Morphological phase‑contrast microscopic observation revealed typical islet‑like cell clusters following 21 days of differentiation. DTZ staining also showed that differentiated ...Continue Reading
References
Apr 2, 1999·Science·M F PittengerD R Marshak
Apr 24, 2001·Endocrinology·X WangJ M Egan
Dec 12, 2002·Molecular Biology of the Cell·Patricia A ZukMarc H Hedrick
Mar 18, 2003·Endocrinology·Hongxiang HuiRiccardo Perfetti
Mar 23, 2004·Laboratory Investigation; a Journal of Technical Methods and Pathology·Seh-Hoon OhBryon E Petersen
Sep 21, 2004·World Journal of Gastroenterology : WJG·Li-Bo ChenLian Yang
Mar 31, 2005·Biochemical and Biophysical Research Communications·Søren BregenholtJacob S Petersen
Apr 1, 2006·Endocrinology·Li-Xin LiPatricia L Brubaker
Jun 17, 2006·Current Opinion in Pharmacology·Mauro KramperaFrancesco Annunziato
Feb 8, 2007·Arthritis Research & Therapy·Alan TyndallMarc Feldmann
May 15, 2007·Circulation Research·Jeffrey M GimbleBruce A Bunnell
Jul 31, 2007·World Journal of Gastroenterology : WJG·Xiao-Hong WuChao Liu
Nov 3, 2007·Neurochemistry International·Dan JiNeville N Osborne
Mar 8, 2008·Experimental Cell Research·Danièle NoëlBéatrice Cousin
Apr 29, 2008·Stem Cells and Development·Lisha LiHanxin Zhou
Jun 3, 2008·Translational Research : the Journal of Laboratory and Clinical Medicine·Feng GaoFu-Jun Li
Feb 24, 2009·Diabetes & Metabolism·L VijaJ Larghero
Apr 22, 2009·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Yu-Hua ZhangYing-Mao Gao
Nov 10, 2009·Diabetes Research and Clinical Practice·J E ShawP Z Zimmet
Jun 21, 2011·PloS One·Vikash ChandraRamesh R Bhonde
Jan 4, 2013·Diabetes Care·UNKNOWN American Diabetes Association
Dec 29, 2012·Journal of Physiology and Biochemistry·P Rahnamay MoshtaghAli M Sharifi
Aug 21, 2013·PloS One·Rui WeiTianpei Hong
Nov 22, 2013·Stem Cell Research·Jennifer E BruinTimothy J Kieffer
Dec 10, 2013·The Journal of Nutritional Biochemistry·Soo Jin YangCheol-Young Park
Apr 1, 2014·Biochemical and Biophysical Research Communications·Kiyoshi TakaharaHaruhito Azuma
Aug 26, 2014·Biochemical and Biophysical Research Communications·Dongyun GaoXiaobing Fu
Citations
Aug 23, 2018·International Journal of Molecular Medicine·Rui WangXining Pang
Oct 19, 2019·Tissue Engineering and Regenerative Medicine·Jianye ZhangShengtian Zhao
Sep 4, 2020·Stem Cell Reviews and Reports·Mohamed A GhoneimMahmoud M Zakaria
Jan 3, 2019·Molecular Pharmaceutics·Alberto Cañibano-HernándezJosé Luis Pedraz