Induction, isolation, and characterization of two laccases from the white rot basidiomycete Coriolopsis rigida.

Applied and Environmental Microbiology
Mario C N SaparratMaría Jesús Martínez

Abstract

Previous work has shown that the white rot fungus Coriolopsis rigida degraded wheat straw lignin and both the aliphatic and aromatic fractions of crude oil from contaminated soils. To better understand these processes, we studied the enzymatic composition of the ligninolytic system of this fungus. Since laccase was the sole ligninolytic enzyme found, we paid attention to the oxidative capabilities of this enzyme that would allow its participation in the mentioned degradative processes. We purified two laccase isoenzymes to electrophoretic homogeneity from copper-induced cultures. Both enzymes are monomeric proteins, with the same molecular mass (66 kDa), isoelectric point (3.9), N-linked carbohydrate content (9%), pH optima of 3.0 on 2,6-dimethoxyphenol (DMP) and 2.5 on 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), absorption spectrum, and N-terminal amino acid sequence. They oxidized 4-anisidine and numerous phenolic compounds, including methoxyphenols, hydroquinones, and lignin-derived aldehydes and acids. Phenol red, an unusual substrate of laccase due to its high redox potential, was also oxidized. The highest enzyme affinity and efficiency were obtained with ABTS and, among phenolic compounds, with 2,6-dime...Continue Reading

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Citations

Jan 24, 2013·Antonie van Leeuwenhoek·Martin RühlUrsula Kües
Jun 21, 2006·Biotechnology Letters·Jose GómezM Angeles Sanromán
Jun 11, 2009·Applied Biochemistry and Biotechnology·Daljit Singh Arora, Rakesh Kumar Sharma
Sep 29, 2011·Applied Biochemistry and Biotechnology·Hui-Ying ChenShan-Jing Yao
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Apr 16, 2013·Preparative Biochemistry & Biotechnology·Amado I Grandes-BlancoMartha D Bibbins-Martínez
Apr 21, 2009·Applied and Environmental Microbiology·Víctor Gómez-ToribioFrancisco Guillén
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Feb 13, 2014·Journal of Industrial Microbiology & Biotechnology·Mario C N SaparratMaría J Martínez
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Mar 1, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Rehman JavaidMuhammad Ferhan
Aug 4, 2019·Chemosphere·María I TroncozoMario C N Saparrat

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