PMID: 9443600Jan 27, 1998Paper

Induction of 12-lipoxygenase expression by phorbol 12-myristate 13-acetate in human epidermoid carcinoma A431 cells

Biochimica Et Biophysica Acta
Y W LiawW C Chang

Abstract

Phorbol 12-myristate 13-acetate (PMA) increased the expression of 12-lipoxygenase activity and mRNA in a time-dependent manner in human epidermoid carcinoma A431 cells. The increase of 12-lipoxygenase was accompanied by the increase in protein level in microsomes prepared from A431 cells. The PMA-induced expression of 12-lipoxygenase activity and mRNA was inhibited by the treatment of cells with a protein kinase C inhibitor GF 109203X. Promoters of different DNA lengths for human 12-lipoxygenase gene were used to prepare the luciferase fusion vectors. These plasmid constructs were transiently transfected into A431 cells. Following treatment of PMA for 18 h, a 4- to 5-fold increase in luciferase reporter activity was observed in plasmids with the 5'-flanking region length of -951 bp and that of -224 bp upstream from translation starting site. A time-dependent induction of luciferase activity by PMA was found to parallel the PMA-induced enzyme activity and mRNA expression. Transient transfection with a series of 5'-deletion constructs showed that the 5'-flanking region spanning from -224 to -100 bp from translation starting site played an important role for PMA response. Gel mobility shift assay and site-directed mutagenesis indi...Continue Reading

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Citations

Nov 16, 2002·Prostaglandins & Other Lipid Mediators·Tanihiro Yoshimoto, Yoshitaka Takahashi
Oct 2, 2003·Prostaglandins & Other Lipid Mediators·Wen-Chang Chang
Jul 29, 2011·Neuropsychopharmacology : Official Publication of the American College of Neuropsychopharmacology·Chi-Tso ChiuDe-Maw Chuang
Sep 6, 2000·Proceedings of the National Academy of Sciences of the United States of America·B K Chen, W C Chang
Feb 16, 2006·Molecular and Cellular Biology·Jan-Jong HungWen-Chang Chang
Nov 28, 2006·Expert Opinion on Drug Metabolism & Toxicology·Margherita Strolin BenedettiEugène Baltes
Aug 12, 1999·Biochemical and Biophysical Research Communications·B K Chen, W C Chang
Jun 27, 2021·Biochimica Et Biophysica Acta. Molecular and Cell Biology of Lipids·Mohammad Golam SabbirPeter Zahradka
Aug 27, 2005·Biochemical and Biophysical Research Communications·Wen-Chang Chang, Ben-Kuen Chen

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