PMID: 9166109May 1, 1997Paper

Induction of 24-hydroxylase cytochrome P450 mRNA by 1,25-dihydroxyvitamin D and phorbol esters in normal rat kidney (NRK-52E) cells

The Journal of Endocrinology
H J ArmbrechtM A Boltz

Abstract

The biologically active form of vitamin D, 1,25-dihydroxyvitamin D (1,25(OH)2D), acts on intestinal, renal, and bone cells to regulate skeletal and mineral metabolism. 1,25(OH)2D also induces 24-hydroxylase activity in these target cells. The 24-hydroxylase hydroxylates 1,25(OH)2D to 1,24,25-trihydroxyvitamin D and 25(OH)D to 24,25-dihydroxyvitamin D. The production of 1,24,25-trihydroxyvitamin D is thought to be the first step in the inactivation of 1,25(OH)2D by its target tissues. Previous studies have characterized the induction of the 24-hydroxylase by 1,25(OH)2D in clonal cell lines from intestine and bone. The purpose of these studies was to characterize the induction of the 24-hydroxylase by 1,25(OH)2D in the kidney, using the clonal rat renal cell line NRK-52E. 1,25(OH)2D (10(-7)M) increased the mRNA levels for the cytochrome P450 component of the 24-hydroxylase (P450cc24) by sevenfold after 36 h in NRK-52E cells. 1,25(OH)2D increased P450cc24 mRNA levels in a dose-dependent manner with an EC50 of 10(-8) M. In parallel experiments, 1,25(OH)2D significantly increased 24-hydroxylase enzyme activity after 48-72 h. The increase in P450cc24 mRNA induced by 1,25(OH)2D required ongoing transcription and translation and was in...Continue Reading

Citations

Jun 11, 2002·Annual Review of Nutrition·John L OmdahlBrian K May
Dec 11, 2003·American Journal of Physiology. Endocrinology and Metabolism·Frank BarlettaSylvia Christakos

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