PMID: 7029258Sep 1, 1981Paper

Induction of 6-thioguanine resistance in synchronized human fibroblast cells treated with methyl methanesulfonate, N-acetoxy-2-acetylaminofluorene and N-methyl-N'-nitro-N-nitrosoguanidine

Mutation Research
S L HuangM D Waters

Abstract

Chemical induction of 6-thioguanine resistance was studied in synchronized human fibroblast cells. Cells initially grown in a medium lacking arginine and glutamine for 24 h ceased DNA synthesis and failed to enter the S phase. After introduction of complete medium, the cells progressed to the S phase after 16h. DNA synthesis peaked 20 h after removal of nutrient stress and declined. Mutations were induced in S-phase cells by methyl methanesulfonate (MMS), N-acetoxy-2-acetylaminofluorene (NA-AAF) and N-methyl-N'-nitro-N-nitroso-guanidine (MNNG). Chemical treatments resulted in an increase in the absolute number of mutant colonies and in a dose-dependent mutation frequency. In this report, we show that NA-AAF evokes a temporal pattern of mutation in synchronized cells, with such mutations being induced only during the S phase. Evidence indicates that presence of S-phase cells in the treated cultures is a prerequisite for the induction of mutations.

References

Mar 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·S G Kee, J E Haber
Jul 1, 1975·Cell·W Burke, W L Fangman
Jan 1, 1976·Journal of Cellular Physiology·J P ThirionT P Vu
Jan 1, 1976·Mutation Research·P M Aebersold, H J Burki
Jan 21, 1969·Biochimica Et Biophysica Acta·R Braun, H Wili
Jan 18, 1966·Biochimica Et Biophysica Acta·G C Mueller, K Kajiwara
Jun 1, 1960·The Journal of Biophysical and Biochemical Cytology·J H TAYLOR
Jun 1, 1963·Experimental Cell Research·S BADERB B MUKHERJEE
Jul 1, 1976·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·I RaskóL Dallmann

❮ Previous
Next ❯