PMID: 7537463Apr 1, 1995Paper

Induction of A- and D-type cyclins and cdc2 kinase activity during recovery from short-term hyperoxic lung injury

The American Journal of Physiology
K C BuiD Warburton

Abstract

Hyperoxia causes a reproducible pattern of lung injury and repair in rodents, in which proliferation of alveolar epithelial cells (AEC) and fibroblasts is observed during recovery. We postulated that if quiescent cells are stimulated to reenter the cell cycle, then cyclin expression and cyclin-dependent protein kinase activity would be reactivated in AEC during the repair process after hyperoxic lung injury. To test this hypothesis, we exposed adult rats to short-term hyperoxia, followed by recovery for various times in room air. Cellular proliferation in vivo was confirmed by 1) flow cytometric analysis of DNA content (FACS) of freshly isolated AEC and 2) immunohistochemistry of proliferating cell nuclear antigen (PCNA) and bromodeoxyuridine (BrdU) incorporation into DNA on lung sections. The percentage of freshly isolated AEC in S phase and G2/M phase on FACS analysis increased twofold to a maximum of 16.5%, after 48 h in 100% oxygen and 48 h recovery in air. Cyclins A and D and p34cdc2 protein expression were also increased during the recovery period; while p33cdk2 and p34cdk4 increased only slightly. p34cdc2 histone H1 kinase activity, both in whole lung and in AEC, decreased initially after 48 h in oxygen. However, a marke...Continue Reading

Citations

Nov 15, 2000·American Journal of Physiology. Lung Cellular and Molecular Physiology·B DriscollD Warburton
Feb 11, 2003·American Journal of Physiology. Lung Cellular and Molecular Physiology·Richard T LightfootHarry Ischiropoulos
Aug 19, 2003·American Journal of Physiology. Lung Cellular and Molecular Physiology·Raghava ReddyBarbara Driscoll
Jul 21, 2006·Journal of Huazhong University of Science and Technology. Medical Sciences = Hua Zhong Ke Ji Da Xue Xue Bao. Yi Xue Ying De Wen Ban = Huazhong Keji Daxue Xuebao. Yixue Yingdewen Ban·Wenbin LiWei Liu
Jun 28, 2011·American Journal of Respiratory Cell and Molecular Biology·Susan BuckleyDavid Warburton
Feb 3, 1998·American Journal of Respiratory Cell and Molecular Biology·M A O'ReillyJ N Finkelstein
Dec 10, 2002·American Journal of Physiology. Lung Cellular and Molecular Physiology·Meera Nanjundan, Fred Possmayer
Dec 8, 2004·American Journal of Physiology. Lung Cellular and Molecular Physiology·S BuckleyD Warburton
Jun 19, 2007·American Journal of Physiology. Lung Cellular and Molecular Physiology·Alessandra PaganoConstance Barazzone Argiroffo
Feb 5, 2008·American Journal of Physiology. Lung Cellular and Molecular Physiology·S BuckleyD Warburton
Jan 15, 2013·American Journal of Physiology. Lung Cellular and Molecular Physiology·Rachel L ZemansGregory P Downey
Jul 15, 2015·The European Respiratory Journal·Susan BuckleyDavid Warburton
Jul 4, 2001·American Journal of Physiology. Lung Cellular and Molecular Physiology·R L AutenM H Whorton
Sep 13, 2002·American Journal of Physiology. Lung Cellular and Molecular Physiology·S Buckley, D Warburton
Nov 22, 2005·American Journal of Physiology. Lung Cellular and Molecular Physiology·Jooeun LeeBarbara Driscoll
Jul 4, 2001·American Journal of Physiology. Lung Cellular and Molecular Physiology·S BuckleyD Warburton
Jul 4, 2001·American Journal of Physiology. Lung Cellular and Molecular Physiology·M A O'Reilly
May 11, 2002·American Journal of Physiology. Lung Cellular and Molecular Physiology·Randolph H HastingsLeonard J Deftos

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