PMID: 6987652Jan 1, 1980Paper

Induction of a basement membrane glycoprotein in embryonic kidney: possible role of laminin in morphogenesis

Proceedings of the National Academy of Sciences of the United States of America
P EkblomL Saxén

Abstract

The glycoprotein laminin is found exclusively in the basement membranes of adult tissues, not in the mesenchymal stroma. We studied the appearance and distribution of laminin during the early formation of kidney tubules in mouse embryos and in an in vitro transfilter model system. In immunofluorescence using affinity-purified antibodies, the distribution of laminin showed a clear correlation, both spatially and temporally, to the early stages of tubule formation. In vivo, laminin was first detected in a punctate pattern in areas where the pretubular aggregates form; later, it became confined to the basement membranes of the tubules. In experiments in vitro, the nephrogenic mesenchyme was found to form tubules after 12-24 hr of transfilter contact with the inductor. The first laminin spots were found after 12 hr of culture, 24 hr before overt morphogenesis. As the mesenchymal cells began to aggregate and elongate (at 36 hr), laminin was detected in those cells destined to become epithelial, and at 48 hr it was not found in cells remaining in the stroma. In more mature tubules (at 72 hr), laminin was seen as a sharp band in the basement membranes. It is suggested that laminin is involved in the increased cell adhesiveness during ...Continue Reading

References

Jul 8, 1975·Biochemical and Biophysical Research Communications·J S Tkacz, O Lampen
Feb 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·G David, M R Bernfield
Mar 13, 1978·Differentiation; Research in Biological Diversity·I ThesleffL Saxén
Jan 1, 1977·The Journal of Experimental Medicine·R W OrkinR Swarm
Jun 4, 1976·Differentiation; Research in Biological Diversity·J WartiovaaraA Vaheri
Jul 1, 1975·The Journal of Experimental Medicine·E LinderJ Wartiovaara
May 15, 1966·International Journal of Cancer. Journal International Du Cancer·L Saxen, J Wartiovaara

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Citations

Jun 8, 2000·Developmental Dynamics : an Official Publication of the American Association of Anatomists·H Colognato, P D Yurchenco
Feb 1, 1990·The American Journal of Anatomy·M M Kleppel, A F Michael
Jan 1, 1993·Cell Motility and the Cytoskeleton·J S WangK S Zaner
Jan 1, 1986·Anatomy and Embryology·L SaxénE Lehtonen
Jan 1, 1987·Anatomy and Embryology·N Shiojiri, H Katayama
Jul 1, 1987·Pediatric Nephrology : Journal of the International Pediatric Nephrology Association·L Saxén, H Sariola
Jul 1, 1990·Pediatric Nephrology : Journal of the International Pediatric Nephrology Association·E D Avner
Dec 1, 1983·In Vitro·I ThesleffE Ruoslahti
Aug 1, 1982·In Vitro·S G PhillipsD M Phillips
Apr 12, 2007·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Susanne SchéelePeter Ekblom
Jan 1, 1983·Developmental Biology·J M Fitch, T F Linsenmayer
Feb 1, 1983·Cell Differentiation·E Belsky, B P Toole
Aug 1, 1988·Cell Differentiation·K HolmH Sariola
Dec 1, 1982·Biochimica Et Biophysica Acta·J D Aplin, R C Hughes
Nov 1, 1988·Cell Differentiation and Development : the Official Journal of the International Society of Developmental Biologists·L SaxénE Lehtonen
Mar 1, 1989·Cell Differentiation and Development : the Official Journal of the International Society of Developmental Biologists·P Y RescanA Guillouzo
Dec 2, 1990·Cell Differentiation and Development : the Official Journal of the International Society of Developmental Biologists·D Edgar
Mar 1, 1982·Collagen and Related Research·M Rojkind, P Ponce-Noyola
Jun 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·A G BrownellH C Slavkin
Jun 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·D E IngberJ D Jamieson
Jun 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·C J CornbrooksR P Bunge
May 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·P EkblomR Timpl
Sep 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·R PytelaE Ruoslahti
Apr 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·J RothJ Finne
May 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·M TaubH K Kleinman
Feb 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·G R Dressler, E C Douglass
May 1, 1985·The Journal of Cell Biology·C B Knudson, B P Toole
Nov 1, 1987·The Journal of Cell Biology·L I FesslerJ H Fessler

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