Induction of a beta-catenin-LEF-1 complex by wnt-1 and transforming mutants of beta-catenin

Oncogene
E PorfiriP Polakis

Abstract

Signal transduction by beta-catenin involves its posttranslational stabilization and import to the nucleus where it interacts with transcription factors. Recent implications for beta-catenin signaling in cancer prompted us to examine colon cancer cell lines for the expression of LEF-1, a transcription factor that binds to beta-catenin. The analysis of several cell lines revealed the expression of LEF1 mRNA and a constitutive association of the LEF-1 protein with beta-catenin. In contrast to the colon cells, PC12 and 293 cells did not contain a beta-catenin-LEF-1 complex, even though both proteins were detected in cell lysates. In these cells, the association of endogenous LEF1 and beta-catenin was induced by stimulation with the wnt-1 proto-oncogene. The complex formed following transient stimulation with wnt-1 and also persisted in cells stably expressing wnt-1. Ectopic overexpression of beta-catenin in 293 cells also induced the assembly of the beta-catenin-LEF-1 complex and activated gene transcription from a LEF-1-dependent promotor. Expression of mutant oncogenic forms of beta-catenin identified in cancer cells resulted in higher levels of transcriptional activity. The results suggest that a cancer pathway driven by wnt-1,...Continue Reading

Citations

Sep 5, 2002·Journal of Surgical Oncology·Holly L NeibergsJohn S Spratt
Oct 19, 2012·Cancer Metastasis Reviews·Robert L Johnson, James C Fleet
Nov 7, 2001·The American Journal of Pathology·F LinJ H Luo
Jun 26, 1998·Current Opinion in Cell Biology·M Bienz
Aug 9, 2002·Journal of Gastroenterology and Hepatology·Tetsuya SuzukiMasamichi Kojiro
Jan 13, 2000·Proceedings of the Society for Experimental Biology and Medicine·M A Van SteenselP M Steijlen
Oct 3, 2012·Proceedings of the National Academy of Sciences of the United States of America·Andrea AguilarNathalie Spassky
Jun 19, 2003·Journal of the National Cancer Institute·Takashi KumagaiH Phillip Koeffler
Oct 13, 2006·Current Opinion in Gastroenterology·Marian L Waterman
Mar 31, 2011·Acta Physiologica·P WendG A Miranda-Carboni
Jul 1, 2006·Molecular and Cellular Biology·Tony W-H LiMarian L Waterman
Aug 22, 2007·Molecular and Cellular Biology·Hirochika KitagawaLeonard P Freedman
Mar 1, 2000·Molecular Pathology : MP·I S Näthke
May 10, 2011·Circulation. Cardiovascular Genetics·Stéphane ChironDaniel T O'Connor
May 30, 2012·BMC Gastroenterology·Shu-Hong WangTao Tian
Oct 26, 2013·BMC Research Notes·Kerryn L ElliottMaree C Faux
Feb 17, 1999·Proceedings of the National Academy of Sciences of the United States of America·B MannC Hanski
Jan 6, 1999·Proceedings of the National Academy of Sciences of the United States of America·M AokiP K Vogt
Apr 12, 2000·Proceedings of the National Academy of Sciences of the United States of America·S Y LinM C Hung
Jan 1, 2011·Leukemia Research and Treatment·S ThanendrarajanI G H Schmidt-Wolf
Jul 2, 2014·Nature Reviews. Drug Discovery·Michael Kahn
Dec 15, 2010·Translational Oncology·Natacha Entz-WerléFabienne Perrin-Schmitt
Aug 3, 2014·Journal of Experimental & Clinical Cancer Research : CR·Samantha Kaufhold, Benjamin Bonavida
Jun 9, 2004·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Man-Young ChaWang-Shick Ryu
Nov 30, 2006·International Journal of Cancer. Journal International Du Cancer·I-Chun TsaiDavid M Virshup
Nov 4, 2005·Journal of Cellular Biochemistry·Rachel A KahlerJennifer J Westendorf
Apr 10, 2007·International Journal of Cancer. Journal International Du Cancer·Takashi KumagaiH Phillip Koeffler

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Proceedings of the National Academy of Sciences of the United States of America
M ShtutmanA Ben-Ze'ev
Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine
Elif AkisikNejat Dalay
© 2021 Meta ULC. All rights reserved