Induction of a high affinity nitrosamine demethylase in rat liver microsomes by acetone and isopropanol

Chemico-biological Interactions
Y Y TuC S Yang

Abstract

The effects of acetone and isopropanol on the microsomal monooxygenase system have been investigated to study the role of this enzyme system in the metabolism of nitrosamines. Treatment of rats with acetone or isopropanol (2.5-5 ml/kg, i.g.) causes a 3-4.5-fold enhancement in the NADPH-dependent nitrosodimethylamine demethylase (NDMAd) activity. This is accompanied by only moderate increases in the gross cytochrome P-450 (P-450) content and NADPH-cytochrome c reductase activity. Several other monooxygenase activities were increased to different extents from an 8% increase in aryl hydrocarbon hydroxylase to a 261% increase in ethoxycoumarin O-dealkylase activities. Kinetic analysis indicates that a low Km form of NDMAd (Km = 0.07 mM) is induced by these treatments. In the microsomes of the treated rats, this high affinity form becomes predominant, in contrast to control microsomes which possess at least three Km-values for NDMAd. The treatment also enhances the metabolism of nitrosomethylethylamine, nitrosomethylbenzylamine and nitrosomethylaniline although to lesser extents than with nitrosodimethylamine. Several lines of observations suggest that the enhanced NDMAd is due to the induction of one or more specific P-450 isozyme(...Continue Reading

References

May 18, 1978·Biochimica Et Biophysica Acta·C S YangL P Kicha
Jun 27, 1977·Zeitschrift Für Krebsforschung Und Klinische Onkologie. Cancer Research and Clinical Oncology·J C ArcosM F Argus
Jan 1, 1979·Chemico-biological Interactions·D Y LaiJ C Arcos
Jun 1, 1978·Chemico-biological Interactions·I G SipesG Holtzman
Jan 1, 1978·Biochemical Pharmacology·C S YangL P Kicha
Mar 1, 1975·Chemico-biological Interactions·O G Fahmy, M J Fahmy
Sep 1, 1971·Toxicology and Applied Pharmacology·G J Traiger, G L Plaa
Jan 1, 1973·Proceedings of the Society for Experimental Biology and Medicine·I G SipesJ R Gillette
Jan 1, 1967·Advances in Cancer Research·P N Magee, J M Barnes
Jan 1, 1971·Annual Review of Pharmacology·M W Anders
Dec 8, 1980·Life Sciences·D Y Lai, J C Arcos
Dec 15, 1981·Biochemical and Biophysical Research Communications·Y Y TuC S Yang
Mar 1, 1977·Biochimica Et Biophysica Acta·C S YangL P Kicha
Oct 1, 1962·The Biochemical Journal·D F Heath

❮ Previous
Next ❯

Citations

Jan 1, 1984·Journal of Cancer Research and Clinical Oncology·H M PylypiwG W Harrington
Jan 1, 1988·Pharmacology & Therapeutics·C S Yang, J S Yoo
Jan 1, 1990·Pharmacology & Therapeutics·A B Okey
Jan 12, 1988·Biochimica Et Biophysica Acta·S Puntarulo, A I Cederbaum
Jul 21, 1992·Biochimica Et Biophysica Acta·D K Winters, A I Cederbaum
May 1, 1986·Toxicology Letters·E P HayesG Witz
Oct 1, 1985·European Journal of Biochemistry·R O JuvonenM A Lang
Dec 1, 1986·Archives of Biochemistry and Biophysics·C J PattenC S Yang
Jan 1, 1990·Drug Metabolism Reviews·C S YangJ Y Hong
Jan 1, 1989·Drug Metabolism Reviews·J B SchenkmanL V Favreau
Mar 1, 1990·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·H IshizakiC S Yang
Apr 30, 1985·Biochemical and Biophysical Research Communications·C S YangM J Coon
Aug 2, 2011·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Chang ZhouJun-Fang Zhou
Jan 1, 1986·Alcoholism, Clinical and Experimental Research·D R Koop, M J Coon
Mar 1, 1984·Archives of Biochemistry and Biophysics·K W Miller, C S Yang
Nov 1, 1995·Journal of Applied Toxicology : JAT·S Dhillon, R Von Burg
Dec 1, 1984·Journal of Applied Toxicology : JAT·T Mendoza-Figueroa
Jan 1, 1993·Critical Reviews in Toxicology·J L RaucyJ M Lasker
Mar 15, 1988·Biochemical Pharmacology·J J Freeman, E P Hayes
May 3, 2003·Biochimica Et Biophysica Acta·Miklós Péter Kalapos
Nov 15, 1992·Archives of Biochemistry and Biophysics·C J PattenC S Yang
Oct 1, 1985·Archives of Biochemistry and Biophysics·Y Y Tu, C S Yang
Feb 13, 1987·Biochemical and Biophysical Research Communications·J Y HongC S Yang
Aug 15, 1990·Archives of Biochemistry and Biophysics·J Y HongC S Yang

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.