PMID: 7516816Feb 1, 1994Paper

Induction of a low voltage-activated, fast-inactivating Ca2+ channel in cultured bone marrow stromal cells by dexamethasone

Calcified Tissue International
S J PublicoverA J el Haj

Abstract

The production of biochemical markers associated with the osteoblastic phenotype, and accompanying changes in the expression of voltage-operated Ca2+ channels, have been examined in rat bone marrow stromal cell cultures treated with dexamethasone (10(-8) M). Whole cell clamp analysis of voltage-operated Ca2+ channels in control cultures (using Ba2+ as the charge carrier) revealed primarily a high voltage-activated (HVA), slowly inactivating current, which was enhanced two- to threefold by treatment of the cells with Bay K 8644 (300 nM) and inhibited by nifedipine (4 microM). In dexamethasone-treated cultures, the I-V relationship for inward current was shifted to more positive potentials in comparison with control cells. Most cells in these cultures possessed both the HVA current and also a faster inactivating, low-voltage-activated (LVA), nifedipine-resistant current. These two currents could be separated both by nifedipine and by the use of steady state inactivation of the LVA current. The two components of the Ba2+ current varied widely in their relative size. The combination of LVA and HVA currents seen in dex-induced stromal cells resembles records of voltage-operated Ca2+ channels from cultures of calvarial osteoblasts.

References

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Citations

Sep 5, 2002·Journal of Biomedical Materials Research·Danielle C GilibertiKay C Dee
Jun 18, 2009·Cellular and Molecular Neurobiology·Traudy AvilaRicardo Felix
Mar 10, 2004·Biochemical Pharmacology·Jue-L WangChung-R Jan
Apr 19, 2002·Biomaterials·Ying YangHaj Alicia J El
Nov 8, 2001·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·I MathovT Bellido
Jun 13, 2001·Biochemical and Biophysical Research Communications·Y GuS J Publicover
Oct 3, 1999·Medical & Biological Engineering & Computing·A J el HajS J Publicover
Feb 1, 1996·Calcified Tissue International·G P Thomas, A J el Haj
Aug 2, 2005·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Ihor ZahanichUrsula Ravens
Oct 26, 2005·Journal of Cellular Physiology·Megan A ForemanStephen J Publicover
Feb 1, 2006·Basic & Clinical Pharmacology & Toxicology·Li-Ling TsengChung-Ren Jan
Oct 13, 2006·American Journal of Physiology. Cell Physiology·Merzesh MagraNicola Maffulli
Nov 1, 1996·The American Journal of Physiology·C M SantiA Hernández-Cruz
Dec 1, 1996·Calcified Tissue International·K S TsaiS T Twu

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