PMID: 6981184Jan 1, 1981Paper

Induction of a lymphotoxin-like mediator in peripheral blood and synovial fluid lymphocytes by incubation with synovial fluid from patients with rheumatoid arthritis

Rheumatology International
G R BurmesterJ R Kalden

Abstract

A significantly increased spontaneous cell-mediated cytotoxicity (SCMC) has been reported in synovial fluid lymphocytes (SFL) as compared to peripheral blood lymphocytes (PBL) of patients with rheumatoid arthritis (RA) and that of normal controls [1-3]. To determine whether this increased SCMC activity is due to the production of a lymphokine and related to the production of a lymphotoxin(LT)-like mediator, PBL from normal controls and PBL and SFL from RA patients were incubated either with a human melanoma cell line (IGR 3) or with cell-free synovial fluid (SF) from RA patients. The SF and the cell-free supernatants of the different cultures were tested for LT activity by estimation of inhibition of DNA synthesis of HeLa cell monolayers and they were added to a SCMC assay system using normal PBL and IGR 3 as target. In the supernatants from cocultures of either PBL from controls or PBL and SFL from RA patients with IGR 3 cells, there was no significant difference in LT activity. An LT-like mediator was observed in the supernatants of all lymphocytes cocultured with SF, whereas SF alone and supernatants of lymphocytes alone exhibited little or no LT activity. In a control experiment, LT induction was not observed when normal ly...Continue Reading

References

Aug 1, 1977·European Journal of Immunology·J R KaldenJ P Cesarini
Nov 1, 1977·Arthritis and Rheumatism·J SebökH W Wouters
May 1, 1975·Arthritis and Rheumatism·P StastnyM Ziff
Jan 1, 1974·Advances in Clinical Pharmacology·P StastnyM Ziff

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Citations

Jan 1, 1997·Arthritis and Rheumatism·G R BurmesterR W Kinne
Dec 1, 1984·Scandinavian Journal of Immunology·S Al-BalaghiE Möller
Feb 25, 2014·FEBS Letters·Matthew G K BeneschDavid N Brindley
Oct 1, 1984·Annals of the Rheumatic Diseases·K FroebelR D Sturrock
Jan 1, 1987·Scandinavian Journal of Immunology·T Egeland, H Lund

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