Induction of a physical linkage between integrins and the cytoskeleton depends on intracellular calcium in an epithelial cell line

Experimental Cell Research
B NebeJ Rychly

Abstract

In most cases epithelial cells reveal a polarized distribution of integrin receptors in basolateral domains of the plasma membrane. To evaluate the functional state of integrin receptors in these restricted sites we were interested in the physical association of integrins with the cytoskeleton. Basically, we extracted cells with Triton X-100 to obtain detergent insoluble cytoskeleton fractions and used monoclonal antibodies for the detection of integrins linked to the cytoskeleton. We found that no permanent physical integrin-cytoskeleton associations exist in a confluent culture of the hepatocyte cell line mHepR1. However, incubation with anti-integrin antibodies and cross linking with a secondary antibody induced a physical linkage of beta1 as well as of different alpha subunits to the cytoskeleton. The association of integrins with the cytoskeleton was also inducible in suspended cells, which was detected in flow cytometric analyses and indicates that the formation of a physical integrin-cytoskeleton connection is independent of the localization of integrins, cell shape, and adhesion on a substrate. Using the Ca2+ chelators BAPTA-AM and EGTA, we found that intracellular calcium is a necessary prerequisite to induce a connect...Continue Reading

Citations

Mar 29, 2002·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Hagen PommerenkeJoachim Rychly
Mar 3, 2007·Gene·James H-C WangHee-Jeong Im
Jul 9, 2004·British Journal of Haematology·Yolande KroviarskiCaroline Le Van Kim
Jul 30, 1999·Biochemical and Biophysical Research Communications·Z WuC A McCulloch
Aug 14, 2001·Journal of Cellular Biochemistry·R D KimR S Chari
Aug 14, 2012·Archives of Dermatological Research·Chenyu HuangRei Ogawa
May 4, 2000·American Journal of Physiology. Cell Physiology·N X ChenR L Duncan

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