Abstract
Choline, acetylcholine and betaine used as a sole carbon source, effectuate in Ps. aeruginosa an acid phosphatase activity in addition to a cholinesterase activity. Induction of both enzyme activities was repressed by succinate or glucose. Cyclic AMP failed to relieve the repression produced by these compounds. Substrates not related to choline and used as a sole source of carbon, were inefficient to produce induction of both enzymes. The in-vitro action of choline, acetylcholine and betaine on Ps. aeruginosa acid phosphatase and cholinesterase has also been studied. To perform these studies periplasmic extracts obtained by EDTA-lysozyme treatment of the cells grown on choline or betaine as sole source of carbon, were used. Acid phosphatase activity was competitively inhibited by betaine, whereas the inhibition produced by choline and acetylcholine showed competitive and noncompetitive components. Cholinesterase activity was noncompetitively inhibited by betaine. At low acetylthiocholine concentration choline was an inhibitor of cholinesterase, whereas at high substrate concentration choline raised the hydrolysis rate of acetylthiocholine. These findings allow the conclusion that acid phosphatase and cholinesterase are specific...Continue Reading
Citations
Jan 1, 1990·Archives of Microbiology·A E LucchiniC E Domenech
Jan 1, 1995·Current Microbiology·G I LucchesiC E Domenech
Feb 20, 2007·Current Microbiology·Nava Katri, Nechama Gilboa-Garber
Feb 1, 1994·The International Journal of Biochemistry·G I Lucchesi, C E Domenech
Jul 4, 2012·Journal of Bacteriology·Liam F FitzsimmonsMatthew J Wargo
Jul 11, 2002·Journal of Bacteriology·Ana L SerraAngela T Lisa
Oct 8, 2013·Journal of Bacteriology·Ken J HampelMatthew J Wargo
Jul 8, 2015·Chemistry and Physics of Lipids·Tatiana KondakovaCécile Duclairoir Poc
Mar 24, 1992·FEBS Letters·C E DomenechM N Garrido
Oct 16, 2018·MicrobiologyOpen·Jennifer BreischBeate Averhoff
Aug 1, 1991·FEMS Microbiology Letters·C E DomenechT A Lisa
Aug 10, 2017·Microbiology·Diego Germán SánchezAngela Teresita Lisa
Jun 22, 2016·Annals of the New York Academy of Sciences·Qinggeng ZhuangChristopher M Hadad
May 22, 1998·FEMS Microbiology Letters·G I LucchesiC E Domenech