PMID: 6406829Jan 1, 1983Paper

Induction of acid phosphatase and cholinesterase activities in Ps. aeruginosa and their in-vitro control by choline, acetylcholine and betaine

Molecular and Cellular Biochemistry
T A LisaCarlos E Domenech

Abstract

Choline, acetylcholine and betaine used as a sole carbon source, effectuate in Ps. aeruginosa an acid phosphatase activity in addition to a cholinesterase activity. Induction of both enzyme activities was repressed by succinate or glucose. Cyclic AMP failed to relieve the repression produced by these compounds. Substrates not related to choline and used as a sole source of carbon, were inefficient to produce induction of both enzymes. The in-vitro action of choline, acetylcholine and betaine on Ps. aeruginosa acid phosphatase and cholinesterase has also been studied. To perform these studies periplasmic extracts obtained by EDTA-lysozyme treatment of the cells grown on choline or betaine as sole source of carbon, were used. Acid phosphatase activity was competitively inhibited by betaine, whereas the inhibition produced by choline and acetylcholine showed competitive and noncompetitive components. Cholinesterase activity was noncompetitively inhibited by betaine. At low acetylthiocholine concentration choline was an inhibitor of cholinesterase, whereas at high substrate concentration choline raised the hydrolysis rate of acetylthiocholine. These findings allow the conclusion that acid phosphatase and cholinesterase are specific...Continue Reading

Citations

Jul 11, 2002·Journal of Bacteriology·Ana L SerraAngela T Lisa
Oct 8, 2013·Journal of Bacteriology·Ken J HampelMatthew J Wargo
Jul 8, 2015·Chemistry and Physics of Lipids·Tatiana KondakovaCécile Duclairoir Poc
Oct 16, 2018·MicrobiologyOpen·Jennifer BreischBeate Averhoff
Jun 22, 2016·Annals of the New York Academy of Sciences·Qinggeng ZhuangChristopher M Hadad
May 22, 1998·FEMS Microbiology Letters·G I LucchesiC E Domenech

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bacterial Cell Wall Structure (ASM)

Bacterial cell walls are made of peptidoglycan (also called murein), which is made from polysaccharide chains cross-linked by unusual peptides containing D-amino acids. Here is the latest research on bacterial cell wall structures.

Bacterial Cell Wall Structure

Bacterial cell walls are made of peptidoglycan (also called murein), which is made from polysaccharide chains cross-linked by unusual peptides containing D-amino acids. Here is the latest research on bacterial cell wall structures.