Induction of adhesion-dependent signals using low-intensity ultrasound.

Journal of Visualized Experiments : JoVE
James RoperMark D Bass

Abstract

In multicellular organisms, cell behavior is dictated by interactions with the extracellular matrix. Consequences of matrix-engagement range from regulation of cell migration and proliferation, to secretion and even differentiation. The signals underlying each of these complex processes arise from the molecular interactions of extracellular matrix receptors on the surface of the cell. Integrins are the prototypic receptors and provide a mechanical link between extracellular matrix and the cytoskeleton, as well as initiating some of the adhesion-dependent signaling cascades. However, it is becoming increasingly apparent that additional transmembrane receptors function alongside the integrins to regulate both the integrin itself and signals downstream. The most elegant of these examples is the transmembrane proteoglycan, syndecan-4, which cooperates with α(5)β(1)-integrin during adhesion to fibronectin. In vivo models demonstrate the importance of syndecan-4 signaling, as syndecan-4-knockout mice exhibit healing retardation due to inefficient fibroblast migration. In wild-type animals, migration of fibroblasts toward a wound is triggered by the appearance of fibronectin that leaks from damaged capillaries and is deposited by macr...Continue Reading

References

Feb 13, 2001·The Journal of Clinical Investigation·F EchtermeyerP Goetinck
Aug 29, 2006·Progress in Biophysics and Molecular Biology·Lutz Claes, Bettina Willie
Jan 17, 2009·The Journal of Biological Chemistry·Claire M MahoneyMark D Bass

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Citations

Mar 20, 2014·Ultrasound in Medicine & Biology·Yaxin HuAlfred C H Yu
Oct 29, 2015·Journal of Athletic Training·Justin H RigbyDavid O Draper
Apr 14, 2020·Journal of Biomechanical Engineering·Jormay LimJaw-Lin Wang
Sep 23, 2020·Genes·Beatriz de LucasBeatriz G Gálvez
Nov 12, 2018·Medical & Biological Engineering & Computing·Hritwick BanerjeeHongliang Ren

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Methods Mentioned

BETA
Pull-down
immunoprecipitation

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