Induction of an activation antigen on human endothelial cells in vitro

European Journal of Immunology
J F LeeuwenbergW A Buurman

Abstract

This study describes the expression characteristics of a cell membrane protein recognized by a monoclonal antibody ENA1, which was obtained by immunizing mice with human umbilical vein endothelial (HUVE) cells cultured with a mixture of interleukin 1 and tumor necrosis factor-alpha. The expression of this ENA1 antigen could also be induced by lipopolysaccharide and phorbol esters. Expression was only demonstrated on HUVE cells and human umbilical arterial endothelial cells, pretreated with one or with a mixture of these reagents. No expression was detected on human fibroblasts, renal epithelial cells or on mesothelial cells derived from omental tissue, either pretreated or not with the aforementioned inducers of the antigen. Furthermore, no reactivity was observed with either polymorphonuclear cells, peripheral blood lymphocytes or the monocytic cell line U937. Time course experiments revealed that the expression of the ENA1 antigen was time dependent. Maximal expression on HUVE cells was observed after 5 h of incubation with activator, after which a decline in expression occurred. Induction of expression could be completely blocked by the mRNA synthesis inhibitor actinomycin D and the protein synthesis inhibitor cycloheximide,...Continue Reading

References

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Citations

Mar 1, 1992·Scandinavian Journal of Immunology·J F LeeuwenbergW A Buurman
Nov 1, 1992·Molecular Biology Reports·J R Westphal, R M de Waal
Oct 6, 1998·Cell Adhesion and Communication·G H HersmannR Bräuer
Nov 17, 2001·Vision Research·Z Pizlo
Feb 2, 2010·Pathologie-biologie·F RavatN Sens
Oct 29, 2017·Journal of Cellular Biochemistry·Ludmila B BuravkovaAnatoly I Grigoriev
Sep 1, 1995·European Journal of Immunology·P PåhlssonA Lundblad
Jun 1, 1993·The Journal of Pathology·A C van Dinther-JanssenC J Meijer
Jul 1, 1992·Biotechnic & Histochemistry : Official Publication of the Biological Stain Commission·E F SmeetsW A Buurman
Oct 1, 1992·European Journal of Immunology·E J von AsmuthW A Buurman
Sep 11, 1996·The American Journal of Physiology·C V SerranoM C Capogrossi
Apr 1, 1991·Resuscitation·G SchlagS Hallström
Jan 1, 1993·The Journal of Investigative Dermatology·J R WestphalR M de Waal
Dec 1, 1992·Differentiation; Research in Biological Diversity·S KlothW W Minuth

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