PMID: 2497979Jan 1, 1989Paper

Induction of an endogenous tumor necrosis factor in mice by murine recombinant interferon-gamma combined with a lipid A subunit analog (GLA-60) of low toxicity

Cancer Immunology, Immunotherapy : CII
I SaikiI Azuma

Abstract

We have investigated the endogenous production of a serum cytotoxic factor when recombinant interferon-gamma (rIFN-gamma) is combined with synthetic lipid A subunit analogs of low toxicity (GLA compounds). The cytotoxic activity of the serum was measured by the crystal violet staining method with L929 cells as a target. Intravenous administration of rIFN-gamma followed by intravenous administration of lipopolysaccharide induced the endogenous production of a cytotoxic factor in the serum. The priming effect of rIFN-gamma appeared immediately and persisted for approximately 20 h after the injection. Administration of lipopolysaccharide as a trigger enhanced the production of the cytotoxic factor in the serum maximally 2 h after the injection. The cytotoxic activity in the serum was completely inhibited by anti-(mouse tumor necrosis factor) (TNF) antibody. A synthetic lipid A subunit analog (GLA-60), which is much less toxic in its endotoxin activities than lipopolysaccharide or synthetic lipid A (compound 506), induced the endogenous production of serum TNF in rIFN-gamma-primed mice. GLA-60 entrapped within liposomes induced the production of serum TNF in rIFN-gamma-primed mice more effectively than GLA-60 solubilized in phospha...Continue Reading

Citations

Jan 1, 1992·Cancer Immunology, Immunotherapy : CII·E KumazawaY Osada
Jan 1, 1990·International Journal of Immunopharmacology·I SaikiI Azuma
Jan 1, 1994·Japanese Journal of Cancer Research : Gann·T SakuraiK Motoyoshi
Oct 1, 1992·Japanese Journal of Cancer Research : Gann·K SatoI Azuma

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