Induction of an epithelial-mesenchymal transition by an in vivo adheron-like complex

Developmental Biology
C H Mjaatvedt, R R Markwald

Abstract

The embryonic vertebrate heart consists of two epithelia: the myocardium and endothelium, separated by the myocardial basement membrane (MBM). The myocardium has been shown to induce endothelial transformation into prevalvular mesenchyme in a temporally and site restricted manner. Previously, we hypothesized that the myocardial-endothelial interaction is mediated in vivo by aggregates of 30-nm particles in the MBM which can be removed by EDTA extraction. These MBM extracts contain fibronectin and other lower Mr proteins and can initiate an epithelial-mesenchymal transition in the AV (atrioventricular canal) endothelium of embryonic chick heart in collagen gel culture. These and other data suggested that the 30-nm multicomponent particles are similar, structurally and compositionally, to multimolecular complexes, termed adherons, secreted by L6 muscle cells in culture. The purpose of this study was to (1) test whether the removal of the 30-nm particles from MBM extracts of embryonic chick hearts would remove the in vitro biological activity and (2) determine if the fractionated MBM extracts can cause AV endothelial cells to follow the same differentiation pathway observed in vivo by monitoring immunohistochemically the cell surf...Continue Reading

References

Jan 1, 1977·The American Journal of Anatomy·R R MarkwaldF J Manasek
Jan 1, 1975·Developmental Biology·R R MarkwaldW N Smith
Feb 1, 1986·The Journal of Cell Biology·G J Cole, L Glaser
Sep 1, 1985·The Journal of Cell Biology·D Schubert, M LaCorbiere
Dec 1, 1987·The Journal of Cell Biology·U HeineM B Sporn
Apr 1, 1987·The Anatomical Record·G T KittenD L Bolender
Oct 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·K L CrossinG M Edelman
Jul 1, 1980·Analytical Biochemistry·B R OakleyN R Morris
Jan 1, 1983·Cold Spring Harbor Symposia on Quantitative Biology·D SchubertC Birdwell
Jul 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·D Schubert, M LaCorbiere
Nov 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·J P ThieryG M Edelman
Jun 1, 1982·Developmental Biology·D H Bernanke, R R Markwald
Jan 1, 1951·Journal of Morphology·V HAMBURGER, H L HAMILTON

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Citations

Aug 1, 1996·The Anatomical Record·H OkagawaM Shimada
Jun 1, 1992·Developmental Dynamics : an Official Publication of the American Association of Anatomists·M WatanabeH Fallah-Najmabadi
Oct 1, 1992·Developmental Dynamics : an Official Publication of the American Association of Anatomists·M NakagawaT K Borg
May 1, 1993·Developmental Dynamics : an Official Publication of the American Association of Anatomists·M L Iruela-ArispeP Bornstein
Oct 1, 1993·Developmental Dynamics : an Official Publication of the American Association of Anatomists·M ChoyP Armstrong
Nov 1, 1994·Developmental Dynamics : an Official Publication of the American Association of Anatomists·D P WitteJ A Harmony
Jun 1, 1992·Molecular Reproduction and Development·R J AkhurstH Slager
Jun 1, 1992·Molecular Reproduction and Development·R B RunyanD L Weeks
Apr 1, 1992·Cell and Tissue Research·H SumidaM Yasuda
Mar 1, 1991·Developmental Biology·M L Iruela-Arispe, E H Sage
Jan 1, 1990·Progress in Growth Factor Research·R J AkhurstF A Millan
Mar 1, 1992·Trends in Cardiovascular Medicine·J RobbinsA Sánchez
Jul 1, 1994·Trends in Cardiovascular Medicine·H S Baldwin, C A Buck
Mar 24, 2010·Toxicological Sciences : an Official Journal of the Society of Toxicology·Alejandro LencinasRaymond B Runyan
Apr 3, 2001·Genetics in Medicine : Official Journal of the American College of Medical Genetics·G M BarlowJ R Korenberg
Mar 27, 1995·Annals of the New York Academy of Sciences·J X HuangR B Runyan
May 1, 1992·American Journal of Respiratory Cell and Molecular Biology·J Roman, J A McDonald
Apr 11, 2009·Toxicologic Pathology·Saija M SavolainenSusan A Elmore
Feb 24, 2011·Development·Juan Antonio GuadixOfelia M Martínez-Estrada
Sep 29, 1995·Experientia·C D Little, B J Rongish
Feb 20, 2003·The Journal of Cell Biology·Josephine M EncisoJoseph A Madri
Apr 18, 2003·Developmental Dynamics : an Official Publication of the American Association of Anatomists·José M Pérez-PomaresAndy Wessels
Dec 17, 2009·Bio Systems·Adrian NeaguGabor Forgacs
Apr 1, 2009·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Russell A NorrisRichard L Goodwin
Jun 16, 2010·Developmental Dynamics : an Official Publication of the American Association of Anatomists·R A NorrisR R Markwald

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