Induction of angiogenesis by murine resistin: putative role of PI3-kinase and NO-dependent pathways
Abstract
Adipose tissue is a highly active endocrine organ, secreting bioactive molecules, adipokines, into the circulation. Obesity results in dysregulated adipokine secretion, contributing to pathophysiologies associated with this disorder, including insulin resistance and cardiovascular disease. To establish whether resistin, a novel bioactive molecule produced by murine adipose tissue, and implicated in insulin resistance in rodents, can induce angiogenic responses in aortic tissues and endothelial cells in vitro, and to investigate the signal transduction pathways involved in these responses. Recombinant murine resistin (5-100 ng ml(-1)) induced sprouting of cellular networks and migration from murine aortic arch explants, primary aortic endothelial cells and in a 'wound healing' model utilising murine b.End5 endothelioma cells. The increased migration and sprouting of endothelial cells, due to resistin, were blocked by wortmannin (100 nM) and LY294002 (10 microM), inhibitors of phosphatidylinositol-3-kinase (PI3K), and accompanied by PI3K-dependent phosphorylation of Akt; moreover, while the changes were not associated with altered production of nitric oxide (NO), resistin-induced angiogenic responses were inhibited by IKK Inhibit...Continue Reading
References
Resistin promotes endothelial cell activation: further evidence of adipokine-endothelial interaction
Citations
Expression and effect of resistin on bovine and rat granulosa cell steroidogenesis and proliferation
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