Induction of antibodies reactive with GM2 ganglioside after immunization with lipopolysaccharides from Camplyobacter jejuni

International Journal of Cancer. Journal International Du Cancer
G RitterL J Old

Abstract

Ganglioside GM2, expressed on the surface of some human cancers, is a promising target for immune therapy, since GM2 antibodies are cytotoxic, can be induced in humans by vaccination, and the presence of GM2 antibodies is associated with a better prognosis in melanoma patients. In our efforts to induce long-lived, cytotoxic GM2 antibodies, we investigated lipopolysaccharides (LPS) containing "GM2-like" oligosaccharides. LPS were prepared from Campylobacter jejuni serotypes O:1, O:23, or O:36 (all sharing the oligosaccharide structure GalNAcbeta1-4Gal(113NeuAc)-Hex with ganglioside GM2), and tested for their ability to induce GM2-reactive antibodies. Immunization of NZW rabbits (2 animals per vaccine) with LPS from C. jejuni serotype O:1 in Freund's adjuvant resulted in production of high-titer IgG antibodies reactive with purified bovine brain GM2 in ELISA, dot-blot immune strains and immune thin-layer chromatography, and with GM2 derived from various human tumors by immune thin-layer chromatography. These rabbit antibodies bound to cancer cell lines expressing GM2 on their cell surface, as determined by mixed hemadsorption assays, mediating strong antibody-dependent cellular cytotoxicity (ADCC) with tumor cells expressing cell...Continue Reading

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Citations

Jul 28, 2004·Proceedings of the National Academy of Sciences of the United States of America·Nobuhiro YukiMitsunori Yamada
Jun 15, 2007·Journal of Neuroimmunology·Amjad A Ilyas, Zi-Wei Chen
Mar 26, 2013·Journal of the Peripheral Nervous System : JPNS·Angie RuppHugh J Willison
Apr 6, 2004·Organic & Biomolecular Chemistry·Søren M AndersenDavid R Bundle

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