PMID: 9005957Jan 15, 1997Paper

Induction of antigen-specific isotype switching by in vitro immunization of human naive B lymphocytes

Journal of Immunological Methods
A ZafiropoulosC A Borrebaeck

Abstract

The use of in vitro immunization technology for the generation of human antigen-specific antibodies has essentially resulted in low affinity IgM antibodies, resembling an in vivo primary immune response. We now describe a detailed reproducible protocol for a two-step in vitro immunization, which yields isotype switched, antigen-specific human antibodies. The immunizing antigen was a 30aa synthetic peptide, containing both a B (15aa V3 peptide of the HIV-1) and a T helper cell epitope (15aa peptide from tetanus toxin). The immunization protocol includes: (i) a selection procedure of donors with a memory T cell response against tetanus toxoid; (ii) immunization of mature naive peripheral B lymphocytes in two distinct phases, involving a primary and a secondary step. None of the donors which were examined after primary immunization showed at any time an IgG anti-V3 specific antibody response, while all the donors showed an IgM response. After the secondary immunization step, anti-V3 antibodies of both IgM and IgG isotypes were detected. The switch frequency event was high among the tested donors (5/8).

References

Dec 1, 1992·European Journal of Immunology·M A Valentine, K A Licciardi
Nov 1, 1988·Immunology Today·C A Borrebaeck
Jun 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·C A BorrebaeckS A Möller
Sep 1, 1981·The Journal of Experimental Medicine·A G BirdK Nilsson
Feb 1, 1994·European Journal of Immunology·D BlanchardJ Banchereau
May 5, 1995·Science·C ArpinY J Liu
May 1, 1993·European Journal of Immunology·K WheelerR Jefferis
Feb 11, 1993·Nature·J P DiSantoG de Saint Basile

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Citations

Nov 1, 2002·Biochemical and Biophysical Research Communications·Stavroula BaritakiElias Krambovitis
Mar 1, 2006·Proceedings of the National Academy of Sciences of the United States of America·Jian LiLuigi Grasso
Sep 6, 2000·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·A AkessonC A Borrebaeck
Feb 26, 2016·BMC Biotechnology·A WijkhuisenM Léonetti
Feb 11, 2011·Biotechnology and Bioengineering·Igor KuzinAndrea Bottaro
Jul 19, 2005·Toxicology Letters·Zaharenia VlataElias Krambovitis
Mar 3, 2007·Biochemical and Biophysical Research Communications·Apostolos K RizosElias Krambovitis
Nov 6, 2002·Journal of Biomedical Materials Research·Stavroula BaritakiElias Krambovitis
Aug 17, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Yoshiro HanyuMieko Kato
Jun 21, 2001·Biochemical and Biophysical Research Communications·A ZafiropoulosE Krambovitis

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