Induction of Antitumor Immunity by Exosomes Isolated from Cryopreserved Cord Blood Monocyte-Derived Dendritic Cells

International Journal of Molecular Sciences
Uyen Thi Trang ThanNhung Thi My Hoang

Abstract

(1) Background: Dendritic cell (DC) vaccination has shown outstanding achievements in cancer treatment, although it still has some adverse side effects. Vaccination with DC-derived exosomes has been thought to overcome the side effects of the parental DCs. (2) Method: We performed the experiments to check the ability of cryopreserved umbilical cord blood mononuclear cell-derived DCs (cryo CBMDCs) and their exosomes to prime allogeneic T cell proliferation and allogeneic peripheral blood mononuclear cell (alloPBMCs) cytotoxicity against A549 lung cancer cells. (3) Results: We found that both lung tumor cell lysate-pulsed DCs and their exosomes could induce allogeneic T cell proliferation. Moreover, alloPBMCs primed with tumor cell lysate-pulsed DCs and their exosomes have a greater cytotoxic activity against A549 cells compared to unprimed cells and cells primed with unpulsed DCs and their exosomes. (4) Conclusion: Tumor cell lysate-pulsed DCs and their exosomes should be considered to develop into a novel immunotherapeutic strategy-e.g., vaccines-for patients with lung cancer. Our results also suggested that cryo umbilical cord blood mononuclear cells source, which is a readily and available source, is effective for generation ...Continue Reading

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Citations

Jun 19, 2020·International Journal of Molecular Sciences·Liliana Czernek, Markus Düchler
Nov 24, 2020·Cancer Control : Journal of the Moffitt Cancer Center·Bui Viet AnhHoang Thi My Nhung
Jan 21, 2021·Journal of Clinical Medicine·Gee-Hye KimSoyoun Um
Jun 3, 2021·International Journal of Molecular Sciences·Sarah Cunningham, Holger Hackstein

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Methods Mentioned

BETA
flow cytometry
transmission electron microscopy
density-gradient centrifugation

Software Mentioned

BD FACSDiva TM
Stata
Navios

Related Concepts

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Cancer Vaccines

Cancer vaccines are vaccines that either treat existing cancer or prevent development of a cancer.