Induction of apoptosis accompanying with G(1) phase arrest and microtubule disassembly in human hepatoma cells by jaspolide B, a new isomalabaricane-type triterpene

Cancer Letters
Shao-Yin WeiWen-Han Lin

Abstract

Jaspolide B is a novel isomalabaricane-type triterpene isolated from sponge Jaspis sp. We investigated its effects on human hepatoma cells in this study. After 48 h of incubation, jaspolide B inhibited the growth of Bel-7402 and HepG2 cells with IC(50) values of 29.1 and 29.5 μM, respectively. Incubation with 0.5 μM of jaspolide B caused time-dependent induction of apoptosis in up to 66.8% of Bel-7402 cells for 48 h, and the induction of apoptosis was confirmed by the enhancement of mitochondrial masses and cell membrane permeability, and nuclear condensation in Bel-7402 and HepG2 cells. Moreover, jaspolide B arrested cell cycle progression at G(1) phase of human hepatoma cells in a dose- and time-dependent manner. In addition, treatment of the compound caused dose-dependent disassembly of microtubule cytoskeleton in Bel-7402 cells at indicated concentrations, and this effect being similar but weaker than that of colchicine, a well-known microtubule-disassembly agent. We conclude that the anti-cancer effect of jaspolide B relates to the apoptosis induction, cell cycle arrest and microtubule disassembly, and these multiple mechanisms of jaspolide B, especially the induction of apoptosis, open interesting perspectives for further...Continue Reading

References

Jul 4, 1990·Journal of the National Cancer Institute·P SkehanM R Boyd
Aug 1, 1988·Pflügers Archiv : European journal of physiology·J HeschelerW Trautwein
Dec 1, 1988·Journal of Muscle Research and Cell Motility·A Takai
Jun 1, 1995·Current Opinion in Cell Biology·W C Earnshaw
Apr 2, 1998·Experimental Cell Research·S Camilleri-BroëtD Hockenbery
Apr 29, 1998·Annual Review of Physiology·G KroemerM Resche-Rigon
Aug 28, 1998·Science·D R Green, J C Reed
Aug 14, 1999·Cancer Chemotherapy and Pharmacology·A A GeldofG T Faircloth
Mar 30, 2001·Journal of Natural Products·K M MeragelmanM R Boyd
Feb 23, 2002·Journal of Natural Products·Deniz TasdemirChris M Ireland
Jul 11, 2002·British Journal of Cancer·A Carnero
Aug 22, 2002·Current Cancer Drug Targets·Angela CasiniLaudiu T Supuran
Dec 8, 2004·Mini Reviews in Medicinal Chemistry·Mohd N Islam, Magdy N Iskander
Oct 26, 2005·Biochemical Society Transactions·C W Gourlay, K R Ayscough
Nov 12, 2005·Pharmacology·Shuichiro HayashiYuichi Majima
Jan 6, 2006·Chemical & Pharmaceutical Bulletin·Shengan TangWenhan Lin
Jul 22, 2010·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Alessia MontagnoliFrancesco Colotta

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Citations

Sep 26, 2009·Natural Product Reports·Reen-Yen KuoKuo-Hsiung Lee
Oct 14, 2017·Marine Drugs·Cinzia CalcabriniCarmela Fimognari
Apr 22, 2016·Zeitschrift Für Naturforschung. C, a Journal of Biosciences·Wei-Guo XuSheng-An Tang
Jul 3, 2021·Marine Drugs·Valentin A Stonik, Sophia A Kolesnikova
Feb 13, 2018·Journal of Natural Products·John H MillerPeter T Northcote
Oct 28, 2019·Journal of Natural Products·Sophia A KolesnikovaValentin A Stonik

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