Induction of apoptosis and necrosis in human peripheral blood mononuclear cells by fatty acid ethyl esters

Alcoholism, Clinical and Experimental Research
Khaled AlhomsiMichael Laposata

Abstract

Fatty acid ethyl esters (FAEE), nonoxidative products of ethanol metabolism, are formed by the esterification of fatty acids and ethanol. Alcoholic subjects have high levels of FAEE in the circulation as well as in organs and tissues, especially those most often damaged by ethanol abuse. Our previous studies showed a significant synthesis of FAEE by human mononuclear cells within seconds of exposure to physiologic doses of ethanol. In addition, FAEE inhibited phytohemagglutinin (PHA)-stimulated interleukin-2 production and calcium (Ca(2+)) influx into human mononuclear cells. FAEE also caused a rapid increase in the intracellular cAMP. The mechanism by which alcohol suppresses the immune system remains undetermined. To evaluate the morphological and physiological effects of FAEE on human mononuclear cells and to study the impact of FAEE on cell viability. Mononuclear cell fractions of human white blood cells (WBC) were incubated with physiological doses (25 and 50 microM) of ethyl oleate, a representative FAEE, for 15, 30, 60, 120 or 180 minutes. Morphological changes were evaluated by light and transmission electron microscopy (TEM). Lactate dehydrogenase (LDH) release was measured as a physiological indicator of necrosis. Phy...Continue Reading

References

Feb 15, 1979·Experientia·G P YoungF J Dudley
Jan 1, 1977·Acta Medica Scandinavica·M MyrhedL E Böttiger
Jun 1, 1992·Alcoholism, Clinical and Experimental Research·G G MeadowsD S Singer
Aug 1, 1990·Alcoholism, Clinical and Experimental Research·T R JerrellsM J Eckardt
Aug 1, 1983·The Journal of Clinical Investigation·L G Lange, B E Sobel
Apr 1, 1993·Alcoholism, Clinical and Experimental Research·S J Ewald, H Shao
Apr 1, 1996·Alcoholism, Clinical and Experimental Research·N P GorskiM Laposata
Jun 1, 1997·Alcoholism, Clinical and Experimental Research·D A BirdM Laposata
Feb 5, 2000·Alcohol and Alcoholism : International Journal of the Medical Council on Alcoholism·G Szabo
Mar 30, 2002·Alcoholism, Clinical and Experimental Research·Sujata KelkarShirish S Barve
Nov 29, 2002·Frontiers in Bioscience : a Journal and Virtual Library·Catherine A Best, Michael Laposata
Feb 27, 2003·Immunology·Aditi A KapasiPravin C Singhal
Dec 8, 2004·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Hikmet Hakan AydinYücel Batur
Jun 24, 2006·Alcoholism, Clinical and Experimental Research·Khaled Alhomsi, Michael Laposata
Jan 1, 1998·Addiction Biology·M Laposata

❮ Previous
Next ❯

Citations

Dec 25, 2009·Applied Microbiology and Biotechnology·Annika RöttigAlexander Steinbüchel
Nov 19, 2013·Cardiovascular Journal of Africa·Maretha Opperman, Spinnler Benade
Feb 24, 2015·Alcoholism, Clinical and Experimental Research·Sowmya S MohanTheresa W Gauthier
Apr 23, 2010·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Bo YuanShuliang Lu
Oct 19, 2016·IUBMB Life·Christoph HeierRobert Zimmermann

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptotic Caspases

Apoptotic caspases belong to the protease enzyme family and are known to play an essential role in inflammation and programmed cell death. Here is the latest research.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Caspases in Metabolic Diseases

Caspases, the family of cysteine proteases are involved in programmed cell death, but their role in metabolic diseases, inflammation and immunity has been of interested. Discover the latest research on caspases in metabolic diseases here.