Abstract
The aim of the present study was to investigate the effects of an oleanolic acid derivative, a novel antitumor drug, on the growth of SMMC-7721 human hepatocellular carcinoma cells and the underlying mechanism. An MTT assay was performed to determine the cytotoxicity of the oleanolic acid derivative. Cell membrane integrity was assessed using fluorescence microscopy to assess the uptake of annexin V-FITC/propidium iodide (PI). Western blotting was used to detect the apoptosis-associated proteins B cell lymphoma-2 (Bcl-2), Bax, caspase-9 and caspase-3. A spectrophotometer was used to analyze the intracellular adenosine triphosphate (ATP) expression level. The loss of mitochondrial membrane potential was detected by performing the JC-1 assay. ELISA was used to evaluate the content of cytochrome c (Cyt-C). The oleanolic acid derivative reduced the cell viability of SMMC-7721 cells in a dose- and time-dependent manner. The half maximal inhibitory concentration values of the oleanolic acid derivative in SMMC-7721 cells at 24, 48 and 72 h were 26.80, 11.85, and 6.66 µM, respectively. The antiapoptotic-protein Bcl-2 was downregulated, and the proapoptotic protein Bax was upregulated following treatment with the oleanolic acid derivati...Continue Reading
References
Dec 9, 1997·Cell·P LiX Wang
Feb 28, 1998·The EMBO Journal·E Bossy-WetzelD R Green
Apr 17, 1999·The Journal of Biological Chemistry·H ZouX Wang
Sep 16, 1999·FEBS Letters·A BobbaE Marra
Feb 25, 2000·The International Journal of Biochemistry & Cell Biology·K Kuida
Mar 8, 2000·American Journal of Clinical Pathology·I O NgM Ng
Mar 30, 2001·Nature·N JozaJ M Penninger
Apr 12, 2003·Developmental Cell·Swathi ArurDavid K Han
Feb 16, 2005·Apoptosis : an International Journal on Programmed Cell Death·M MourdjevaS Kyurkchiev
May 24, 2005·Cell·Zhenyue HaoTak W Mak
Mar 15, 2006·Plant Physiology·Rosa Anna VaccaErsilia Marra
Dec 27, 2008·Cell·Lorenzo Galluzzi, Guido Kroemer
Oct 31, 2009·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Alessandro VitaleUmberto Cillo
Sep 16, 2010·Molecular Therapy : the Journal of the American Society of Gene Therapy·Sara SamuelJohn Hiscott
Mar 5, 2011·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Jordi BruixUNKNOWN American Association for the Study of Liver Diseases
Jul 16, 2011·International Journal of Cell Biology·Michele A HoustonBarbara G Heerdt
Apr 28, 2012·Gastroenterology·Hashem B El-Serag
Jun 9, 2012·Journal of Applied Toxicology : JAT·Jianteng WeiXiukun Lin
Jul 25, 2012·European Journal of Pharmacology·Ling LiuYihua Zhang
Sep 25, 2012·Cellular Signalling·Qing-you ZhengXing Ai
Oct 8, 2013·PloS One·E Magda BarbuDimitrios P Kontoyiannis
Mar 4, 2014·PloS One·Xu WangDong M Shin
Jul 6, 2014·International Journal of Oncology·Ji Hoon JangJong-Wook Park