Abstract
The effect of taxol (TX) and cisplatin (CDDP), singly or in association, was assessed on two human ovarian cancer cell lines, one sensitive (A2780) and one resistant (A2780 cp8) to CDDP. Cell lines showed a similar sensitivity to TX, whereas different cytotoxicity results were obtained in the two cell lines as a function of TX and CDDP sequence. Specifically, TX followed by CDDP induced simply additive effects in both cell lines, whereas the opposite sequence produced antagonistic effects in A2780 cells and synergistic effects in A2780 cp8 cells. TX, with or without CDDP, induced oligonucleosomal DNA fragmentation typical of the apoptotic process, but the biochemical mechanisms undergoing apoptosis were different in the two cell lines. In fact, in A2780 cells, TX (with or without CDDP) treatment markedly increased p53 as well as p21waf1 protein expression. In A2780 cp8 cells, drug treatment enhanced p53 levels, whereas the expression of p21waf1 was always undetectable at mRNA and protein levels. In the latter cell line, a premature activation of p34cdc2 kinase was observed in correspondence with the drug-induced increase in the S-phase cell fraction. Such an activation was not ascribable to an increase in the overall expression...Continue Reading
Citations
Jun 22, 2000·Cancer·T H WangY K Soong
Jul 6, 2001·Oncogene·P GiannakakouM V Blagosklonny
Sep 20, 2005·Breast Cancer Research : BCR·Wainer ZoliDino Amadori
Mar 6, 2017·International Journal of Oncology·Yaomin WangYi Charlie Chen
Apr 10, 1999·The Journal of Biological Chemistry·S GibsonG L Johnson
Mar 16, 2001·Expert Opinion on Pharmacotherapy·I Sekine, N Saijo
Sep 2, 1999·International Journal of Cancer. Journal International Du Cancer·M V Blagosklonny, T Fojo
Dec 22, 1999·British Journal of Cancer·C F O'NeillL R Kelland
Oct 3, 2002·Japanese Journal of Cancer Research : Gann·Julia K BarAndrzej Ozyhar
Apr 24, 2003·Cancer Chemotherapy and Pharmacology·Seiji SawadaTomohiro Matsumura
Apr 22, 2006·Cancer Gene Therapy·A PataerK K Hunt
Dec 6, 2005·Journal of Chemotherapy·M M M Abdel-LatifJ V Reynolds
Aug 25, 2010·Cancer Chemotherapy and Pharmacology·Murugan KalimuthoSergio Bernardini
Mar 4, 2000·Human Gene Therapy·H SauthoffJ G Hay
Nov 2, 2002·Cancer·Chiun HsuAnn-Lii Cheng
May 18, 2001·Endocrinology·K C ChoiP C Leung
Nov 11, 2015·Scientific Reports·Zhang-Feng ZhongYi-Tao Wang
Jun 1, 2000·Breast Cancer Research and Treatment·A ChaddertonA M Parissenti
Feb 17, 2001·Journal of Cellular Biochemistry·L OrlandiN Zaffaroni
Dec 20, 2005·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Jyotsnabaran HalderAnil K Sood
Mar 29, 2003·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Fiamma ButtittaSilvano Bosari
Aug 11, 2001·Drug Resistance Updates : Reviews and Commentaries in Antimicrobial and Anticancer Chemotherapy·Manish A. Shah, Gary K. Schwartz
Dec 22, 2011·Chemical Research in Toxicology·Punnajit LimJohn Termini